海蒿子含氮有机化学成分研究

Nitrogenous compounds from Sargassum pallidum

目的对海蒿子Sargassum pallidum全草进行含氮有机化学成分研究。方法采用D101大孔吸附树脂柱色谱、正相硅胶柱色谱、凝胶柱色谱、ODS柱色谱、高效液相色谱等方法对含氮有机化学成分进行分离纯化,并根据其理化性质和核磁共振谱、质谱等光谱学技术鉴定所分离化合物的结构。结果从海蒿子全草70%乙醇提取部位分离得到了10个化合物,分别鉴定为(-)-伞形香青酰胺(1)、(-)-栝楼酯碱(2)、(-)-金色酰胺醇酯(3)、核黄素(4)、β-腺苷(5)、2′-Ο-甲氧基尿嘧啶核苷(6)、环(L-丙氨酸-L-脯氨酸)(7)、胸腺嘧啶核苷(8)、1-(β-D-ribofuranosyl)-1H-1,2,4-triazone(9)、2,3-二氢-4(1H)-喹诺酮(10)。结论化合物1、2、4~7、9为首次从马尾藻属植物中分离得到。

Objective To study the nitrogenous compounds of Sargassum pallidum. Methods The compounds were separated and purified by D101 macroporous adsorptive resins, silica gel chromatography, Sephadex LH-20 chromatography, ODS column chromatography, and HPLC methods. Their structures were identified on the basis of physical and chemical properties and spectroscopic data（^1H-NMR, ^13C-NMR, and MS）. Results Ten compounds were isolated from S. pallidum and identified as（-）-anabellamide（1）,（-）-trichosanatine（2）,（-）-aurantiamide acetate（3）, riboflavin（4）, β-adenosine（5）, 2′-Ο-methoxyluridine（6）, cyclo（L-Ala-L-Pro）（7）, thymidine（8）, 1-（β-D-ribofuranosyl）-1H-1,2,4-triazone（9）, and 2,3-dihydro-4（1H）-quinolone（10）. Conclusion Compounds 1, 2, 4—7, and 9 are isolated from the genus Sargassum（Turn.） C. Ag. for the first time.