X线钼靶摄影乳腺癌钙化与ER、PR及c-erbB-2表达的相关性研究

Study on Correlation Between Calcifications of Breast Cancer under X-ray Mammography and Expression of ER,PR and c-erbB-2

目的:探讨乳腺癌钙化灶特点与人表皮生长因子受体-2(c-erbB-2)、孕激素受体(PR)及雌激素受体(ER)的表达水平之间的关系。方法:观察100例乳腺癌患者X线钼靶摄影片中钙化灶的特点,采用免疫组织化学染色方法测定癌组织中免疫组化标志物c-erbB-2、PR及ER的表达水平,将二者结果进行对照研究。结果:100例患者中,55例患者无钙化表现,45例患者有沙砾样微小钙化灶表现。45例钙化灶组的c-erbB-2阳性表达明显高于55例无钙化组,差异有统计学意义(χ~2=5.285,P=0.022);45例有钙化组中,钙化沿乳腺导管走行组的PR阳性表达明显低于钙化不沿乳腺导管走行组,差异有统计学意义(χ~2=3.910,P=0.048);钙化灶总数目>20个组别的c-erbB-2、PR、ER阳性表达均稍高于钙化灶总数目≤20个组别,但差异无统计学意义(P>0.05);单纯钙化组的ER阳性表达明显低于钙化合并肿块组,差异有统计学意义(χ~2=4.377,P=0.036)。结论:乳腺癌钙化灶特点与乳腺癌分子生物学指标(c-erbB-2、PR、ER)之间存在一定相关性,是否有钙化、钙化灶的分布及钙化是否合并肿块存在等可能粗略预测部分生物学指标的表达,为乳腺癌分子生物学行为和疾病的预后判断提供一定依据。

Objective： To explore the relationship between calcification of breast-cancer and the expression of epidermal growth factor receptor-2 （c-erbB-2）, progesterone receptor （PR） and estrogen receptor （ER）. Methods： The characteristics of calcifications of breast cancer was observed with X-ray mammography in 100 cases. All post-surgery tissue samples from the 100-subjects were further processed for immunohistochemical staining to characterize the expression of c- erbB-2, PR and ER. The results were compared. Results： Calcifications were absent in 55 patients and microsand-like calcifications were evident in 45 patients among 100 cases of breast cancer. Based on whether there were calcifications, the expression of c-erbB-2 was significantly higher in with-calcification patients （ x^2 = 5.28, P = 0. 022 ） ; based on whether there were along-duct calcifications, the expression of PR was significantly lower in patients without along-duct calcifications （ x^2 = 3.910, P = 0. 048 ） ; based on whether there were above-20 calcifications, the expression of c-erbB-2, PR and ER were slightly but non-significantly higher in patients with above-20 calcifications （ P 〉 0. 05 ） ; based on wheth- er there were with-lump calcifications, the expression of ER was obviously and significantly lower in patients without lumps （x^2 = 4. 377 ,P = 0. 036）. Conclusion： There is a certain correlation between the characteristics of calcifications and c-erbB-2 expression,PR expression and ER expression of breast cancer. Whether having calcification, the distribution of calcification, and calcifications combined with lumps may roughly predict the expression of some biological indicators, which provides certain basis for the molecular biological behavior and prognosis of breast cancer.