驼奶对急性酒精肝损伤的辅助保护作用

Protective effect of camel milk on acute alcoholic liver injury

目的探讨驼奶对小鼠急性酒精性肝损伤的辅助保护作用。方法60只雄性ICR小鼠随机分为5组,即500、1 000、3 000mg/kg b.wt.等3个剂量组、模型对照组和空白对照组。剂量组分别经口给予相应剂量的驼奶水溶液,模型对照组和空白对照组给予纯净水。连续灌胃30d后,空白对照组继续给予纯净水,其余各组于实验结束时1次给予50%的乙醇(14mL/kg b.wt.)灌胃,禁食16h,以建立小鼠急性肝损伤模型,分别检测各组肝匀浆中甘油三酯(TG)、还原型谷胱甘肽(GSH)、丙二醛(MDA)和血清中极低密度脂蛋白(VLDL)的含量,观察肝脏病变情况并比较。结果灌胃30d后,各剂量组与对照组小鼠体重差异均无统计学意义(P>0.05);模型对照组肝匀浆MDA和TG含量高于空白对照组,肝匀浆GSH和血清中的VLDL含量低于空白对照组(P值均<0.01),急性酒精肝损伤模型成功建立。各剂量组小鼠肝匀浆中MDA含量低于模型对照组,GSH含量高于模型对照组,其中500、1 000mg/kg b.wt.剂量组小鼠肝匀浆MDA、GSH含量,与模型对照组差异均有统计学意义(P值均<0.05);各剂量组小鼠肝匀浆中TG含量低于模型组,差异均有统计学意义(P值均<0.01);各剂量组血清VLDL含量高于模型对照组,3 000mg/kg b.wt.剂量组与模型对照组差异有统计学意义(P<0.05)。与模型对照组相比,各剂量组小鼠肝细胞脂肪变性不同程度减轻,其中1 000mg/kg b.wt.剂量组病变程度与模型对照组差异有统计学意义(P<0.05)。结论实验条件下,驼奶对小鼠急性酒精肝损伤有一定的辅助保护作用。

Objective to investigate the protective effect of camel milk on acute alcoholic liver injury in mice. Methods A total of 60 male ICR mice were randomly divided into 5 groups,including 3 dose groups（ 500,1 000,3 000 mg/ kg b. wt. ）, model control group and blank control group. All mice in dose groups were given camel milk solution while mice in the model control group and blank control group were given pure water via intragastrie administration. After 30 d, the blank control group was given pure water while other groups were given 50 % ethanol （14 mL/kg b. wt. ） by intragastric administration; all mice were subjected to 16 h fasting to establish acute liver injury in mice model. Mice of the above 5 groups were analyzed for concentration of liver homogenate triglyeeride （TG）, reduced glutathione （GSH）, malondiatdehyde （MDA） and serum very low density lipoprotein （VLDL）;in addition, liver lesions were observed and compared. Results After intragastric administration of 30 d,no statistical difference was observed among body weight of mice in dose groups and control groups（P〉0.05）. The concentrations of MDA and TG in liver homogenate of model control group were higher than those of blank control group, the concentrations of GSH in liver homogenate and serum VLDL was lower than those of blank control group （all P〈0.01）, the mice acute alcoholic liver injury was established successfully. Compared to mice in model control group, the MDA concentrations in liver homogenate of mice in all dose groups were lower while the GSH concentrations were higher, among which MDA and GSH concentrations of mice in 500 and 1 000 mg/ kg b. wt. dose group were statistically different （all P〈0.05） ; the mice liver homogenate TG concentrations in all dose groups were lower and serum VLDL were higher （all P〈0. 01） ,among which VLDL of mice in 3 000 dose group was statistically higher than that of model control group （P〈0.05） ; the hepatic steatosis of mice in all dose groups was alleviated, and the pathological changes of the 1 000 mg/ kg b. wt. group were significantly different with that in the model control group. Conclusion Under the experimental conditions, the camel milk has some protective effects on acute alcoholic liver injury in mice.