HPLC法快速测定8种中药中齐墩果酸和熊果酸

Rapid determination of oleanolic acid and ursolic acid in eight kinds of Chinese materia medica by HPLC

目的建立一种快速分析方法,同时测定中药材马鞭草Verbena officinalis、女贞子Ligustrum lucidum、夏枯草Prunella vulgaris、白花蛇舌草Hedyotis diffusa、败酱草Patrinia scabiosaefolia、枇杷叶Eriobotrya japonica、山楂Crataegus pinnatifida、木瓜Chaenomeles Fructus papaya中齐墩果酸和熊果酸的量。方法使用快速溶剂萃取法,采用甲醇作为提取溶剂,静态萃取时间6 min,制备样品溶液,液相色谱分析采用Acclaim C_(30)色谱柱,以乙腈-0.2%醋酸(85∶15)为流动相,体积流量为0.3 mL/min,紫外检测波长为205 nm。结果样品提取时间为10 min,待测物齐墩果酸和熊果酸达到基线分离,且无样品基质干扰,r大于0.999,平均回收率在95.8%～102.7%,本法测定结果与《中国药典》2015年版方法的平均质量分数差异不显著。结论本方法简便、快速,且一法多用,可用于8种中药中齐墩果酸和熊果酸的测定。

Objective To establish a method of rapid analysis for simultaneous determination of oleanolic acid and ursolic acid in Chinese materia medica （CMM） of Verbena oJficinalis, Ligustrum lucidum, Prunella vulgaris, Hedyotis diffusa, Patrinia scabiosaefolia, Eriobotrya japonica, Crataegus pinnatifida, Chaenomeles Fructus papaya. Methods Theanalysesofpreparation were conducted by accelerated solvent extraction （ASE）, methanol was used as solvent extraction, and the static extraction time was 6 min. Separation was carried out on Acclaim C30 Thermo column with acetonitrile and 0.2% acetic acid （85 ： 15） as mobile phase, flow rate was 0.3 mL/min, and UV detection wavelength was set at 205 nm. Results After 10 rain sample extraction time, oleanolic acid and ursolic acid reached baseline separation, and no sample matrix was interfered, the linear correlation coefficient was over 0.999, the average recovery between 95.8% and 102.7%; Compared to Chinese Pharmacopoeia 2015, the determination of average mass fraction difference was 3.7% and 5.8%. Conclusion This method is simple, fast, and suitable for the analysis of these drugs, and it can be used for content determination of oleanolic acid and ursolic acid for eight kinds of CMM.