辣椒炭疽病菌抗啶氧菌酯突变体渗透压敏感性及交互抗性

Osmotic Pressure Sensitivity and Cross-resistance of Colletotrichum gloeosporioides Resistant Mutants to Picoxystrobin

为了探讨辣椒炭疽病菌Colletotrichum gloeosporioides敏感菌株与抗啶氧菌酯突变体对渗透压敏感性的差异,室内诱导获得了C.gloeosporioides对啶氧菌酯的抗性突变体,在此基础上,对其抗感菌株渗透压敏感性及交互抗性进行了研究。结果表明,抗感菌株在供试葡萄糖浓度下均可生长,相比无糖培养基,在含有葡萄糖的培养基上菌落直径显著增加;随着培养基中NaCl浓度的升高,抗感菌株菌丝生长均受到抑制,且敏感菌株与不同抗性水平抗性突变体对高浓度NaCl渗透压均敏感,但渗透压敏感性没有显著差异。啶氧菌酯与嘧菌酯、醚菌酯、多菌灵、咪鲜胺的交互抗性测定结果显示,啶氧菌酯与同属甲氧基丙烯酸酯类杀菌剂的嘧菌酯、醚菌酯之间存在正交互抗性,与多菌灵、咪鲜胺之间均无交互抗性产生。该研究结果可为指导生产中合理用药提供理论依据。

To investigate the osmotic pressure sensitivity of Colletotrichum gloeosporioides picoxystrobin-resistant mutants and sensitive strains, the research induced and obtained C. gloeosporioides resistant mutants to picoxystrobin. On this basis, the osmotic pressure sensitivity and the cxoss-resistance were studied. The results showed that all isolates could grow well on the PDA medium with all of glucose concentrations and the colony diameters increased obviously in the medium containing glucose compared with no glucose. But all isolates grew poor with the increase of NaCl concentration and both resistant and parent sensitive isolates were sensitive to high NaCl concentration. The results of cross-resistance showed that picoxystrobin had positive cross-resistance with azoxystrobin and kresoxim-methyl, while no cross-resistance was detected between picoxystrobin and other type fungicides, such as carbendazim and prochloraz. The research results provide theoretical basis for the guidance of pesticides use in production.