纤维素酶辅助黑曲霉提取果胶

Extraction of Pectin by Aspergillus Niger Assisted with Cellulase

利用纤维素酶辅助黑曲霉提取柠檬皮中的果胶,采用咔唑硫酸法测定提取液中的果胶含量。在单因素实验的基础上,以果胶提取率为指标,利用Box-Behnken设计四因素三水平进行响应面试验,建立各因素与响应值之间的数学模型,确定最佳提取工艺。结果表明,最佳工艺条件为:纤维素酶处理时间1.0 h,处理温度45℃,缓冲液pH=5.0,酶量0.5%,发酵时间24 h,在此工艺条件下,果胶提取率为7.38%。

Pection was extracted by aspergillus niger assisted with cellulase from lemon peel. The content of pectin was measured by carbazole-sulfuric acid method. A Box-Behnken design involving four factors at three levels each was used to determine the optimum extraction conditions using pectin yield as response. The optimum extraction conditions were obtained as follows： cellulase treatment time was 1.0 h, cellulase treatment temperature was 45℃, buffer pH = 5.0, cellulase concentration was 0. 5% and fermentation time was 24 h. Under the optimized conditions, the yield of pectin was 7. 38%.