摘要
建立了高效液相色谱测定番石榴中总维生素C含量的方法。样品经过0.1%草酸溶液提取后,用L-半胱氨酸溶液将脱氢抗坏血酸还原成还原型抗坏血酸后测定L-抗坏血酸总量。采用C_(18)柱(4.6 mm×250 mm,5μm),流动相为0.1%草酸溶液,流速1.0 mL/min,检测波长245 nm。L-抗坏血酸在10.0~500μg/mL范围内线性良好,检出限为5.0 mg/kg,回收率为90%~103%,RSD为0.55%。该方法操作简便,灵敏度高,准确性好,适用于番石榴及其他水果中总维生素C的测定。
The method was developed for the determination of total vitamin C in guava by high performance liquid chromatography. After the sample was extracted in 0. 1% oxalic acid, DAsA was reduced to AsA by L-cysteine, and then the total aseorbic acid was determined. The analytes were separated on a C18 column （4.6 mm×250 mm, 5 μm） using 0. 1% oxalic acid as mobile phase at a flow rate of 1.0 mL/min, and the detection was carried out at a wavelength of 245 nm. L-ascorbic acid had a good linerility in the range of 10. 0 - 500 μg/mL. The determination of limit was 5. 0 mg/kg. The average recoveries were in the range of 90% - 103% accurate, suitable for the determination of total vitamin C
出处
《广州化工》
CAS
2017年第11期136-138,共3页
GuangZhou Chemical Industry
基金
广东省科技计划项目(2013B091604003
2014B070705001)
关键词
番石榴
L-抗坏血酸
总抗坏血酸
高效液相色谱法
guava
L-ascorbic acid
total ascorbic and RSD was 0.55%. This method was simple, sensitive and in guava and other fruits. acid
high-performance liquid chromatography （HPLC）
