运动饮料中糖分的检测

Analysis Sugar in Sports Drink

建立一种用高效液相色谱-蒸发光检测法测定运动饮料中常用的木糖、果糖、葡萄糖、半乳糖、蔗糖、麦芽糖、乳糖、蔗果三糖、蔗果四糖、蔗果五糖等10种糖分的检测方法。样品经乙腈-水提取,过滤膜后上机检测。采用ZORBAX糖分析色谱柱(4.6 mm×250 mm,5μm)分离,以乙腈-水梯度洗脱作为流动相,用蒸发光检测器检测,外标法峰面积定量。结果表明,10种糖组分在0.01 g/100 m L^0.50 g/100 m L浓度范围内线性良好,相关系数r2为0.996~0.999,检出限为0.02 mg/m L^0.1 mg/m L,定量限为0.06 mg/m L^0.3 mg/m L,加标回收率达到90.2%~98.5%。

A rapid and effective method was established for the determination of ten sugars （xylose,fructose, glucose, galactose, sucrose, maltose, lactose, 1 -kestose, nystose, 1 F- Fructofuranosyl nystose ）in sports drink by high performance liquid chromatography-ELSD detection method. The samples were extracted by acetonitrile- water, and detected on the machine after filtering membrane. The separation of targeted compound was per- formed on a ZORBAX sugar analysis chromatography column （4.6 mm×250 mm,5μm） using acetonitrile-wa- ter as mobile phase, with ELSD detector and external standard method peak area quantification. The linear range of ten kinds sugar was in the range of 0.01 g/100 mL-0.50 g/100 mL with a correlation coefficient of 0.996-0.999. The detection limit was 0.02 mg/mL-0.1 mg/mL and the quantitative limit was 0.06 mg/mL- 0.3 mg/mL. The recovery rate was 90.2 %-98.5 %.