宁夏贺兰山东麓酿酒酵母分离筛选及菌株鉴定

Isolation and Identification of Saccharomyces cerevisiae Strain of East Helan Mountain Area in Ningxia

为进一步探究宁夏贺兰山东麓酿酒葡萄酵母菌的多样性,更好开发利用葡萄产区酵母菌资源。从宁夏贺兰山东麓的西夏王陵、红寺堡、玉泉营、青铜峡、张裕十五世酒庄地区的葡萄园土壤、葡萄浆果表皮以及葡萄自然发酵过程中进行酵母菌的分离筛选,对得到的122株酵母菌株运用WL营养琼脂培养基进行初步分类,同时结合26S r DNA D1/D2区序列分析。共鉴定出克鲁维毕赤酵母(Pichia kluyveri)、美极梅奇酵母(Metschnikowia pulcherrima)、葡萄汁有孢汉逊酵母(Hanseniaspora uvarum)、酿酒酵母(Saccharomyces cerevisiae)、大隐球酵母(Cryptococcus magnus)共5种酵母菌。进而初步确定宁夏贺兰山东麓酿酒葡萄产区酵母菌的主要种类,为地方特色葡萄酒酵母菌的筛选和选育提供理论基础和研究依据。

In order to further explore the diversity of Saccharomyces cerevisiae of Helan Mountain Area in Ningxia and realize better development and utilization of Saccharomyces cerevisiae resources, the wine grape regions of Mausoleum of Xixia Dynasty, Hongsibu, Yuquanying, Qingtongxia and Chateau Changyu Moser XV were taken as the research object. The Saccharomycetes in the soil was isolated, screened and observed in the natural fermentation process of grape berries epidermis and the fruit. The 122 Saccharomycetes strainswere pre- liminarily classified based on WL nutrient agar, and 26S rDNA D1/D2 sequence analysis was conducted. Totally, 5 kinds of Saccharomycetes were identified in this study, including Pichia kluyveri, Metschnikowia pul- cherrima, Hanseniaspora uvarum, Saccharomyces cerevisiae and Cryptococcus magnus. Thus the main species of Saccharomycetes in the wine grape region of Helan Mountain Area in Ningxia was preliminarily determined, which provides theoretical foundation and research basis for the screening and breeding of local Saccharomyces cerevisiae.