核桃内种皮提取物对小鼠脑损伤的改善作用

Protective Effect of Walnut Pellicle Extracts on Cerebral Injury in Mice

研究核桃内种皮多酚提取物对D-半乳糖致衰小鼠脑组织氧化损伤程度的调节。选取ICR雄性小鼠60只,随机分为5组,每组12只,对模型对照组和剂量组小鼠采取颈部皮下注射D-半乳糖[120 mg/(kg·bw·d)]制造衰老模型,低、中、高剂量组分别灌胃200、400、800 mg/(kg·bw·d)的核桃内种皮多酚提取物,模型对照组灌胃等量的双蒸水。给药7周后,通过旷场试验、紧绳实验和平衡木实验检测每组小鼠间的行为学差异。8周后,取脑组织,测定脑组织匀浆中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活力和总抗氧化能力(T-AOC)及丙二醛(MDA)的含量。结果表明,与模型对照组相比,核桃内种皮多酚提取物可显著提高小鼠的运动和探索能力,增强小鼠的兴奋性和对环境的适应性。同时,脑组织中SOD、GSH-Px活力和T-AOC含量较模型组有显著升高。这表明核桃内种皮多酚提取物可减轻D-半乳糖导致的行为迟钝,并能减轻小鼠脑部的氧化损伤。

To observe the effects of phenolic extracts from walnut （Juglans regia L. ） kernel pellicle （EWP） by- product on cerebral injury aging mice induced by D-galactose. Male ICR mice （n=60） were randomly divided into five groups of 12 each. Hypodermic injection of D-gal at the dose of 120 mg/（kg· bw· d）, low, middle and high groups gived EWP by 200,400, 800 mg/（kg· bw· d） respectively, while the control group garage equiva- lent normal saline. After 7 weeks, the behavior changes were investigated by open-field test, tightrope test and balancing beam test between dosage groups and normal controls. After 8 weeks, the contents of malonialdehyde （MDA）, the activities of superoxide dismutase （SOD）, catalase （CAT）, glutathione peroxidase （GSH-Px） and total antioxidant capacity （T-AOC） in brain were observed. The result showed that EWP could significantly improve the mice＇s ability to exercise and explore, enhance the excitability and the adaptability to the environ- ment. At the same time, it significantly increased activities of the antioxidant enzymes by enhanced activities of SOD, GSH-Px, T-AOC as compared to model group. This indicated that EWP could reduce the action retarda- tion induced by D-galactose, and could reduce the oxidative damage in the brain of mice.