急性白血病患者外周血CD4^+CD25^+Foxp3^+调节性T细胞检测及其对细胞凋亡的作用

Peripheral Blood Cd4^+Cd25^+Foxp3^+ Regulatory T Cells Detection And Its Effect On Cell Apoptosis In Acute Leukemia

目的研究CD4^+CD25^+Foxp3^+调节性T细胞在急性白血病患者外周血中的检测,并探讨其对细胞凋亡的作用。方法将65例急性白血病患者(包括急性淋巴细胞性白血病33例和急性髓系白血病32例)分为未缓解组(28例)和缓解组(37例),25例健康志愿者作为对照组。根据是否合并感染又分为合并感染组(39例)和未合并感染组(26例)。采用细胞内染色的流式细胞术及荧光定量PCR的方法,分别在蛋白质和mRNA水平检测Foxp3表达,并与正常对照组进行比较。同时,利用荧光定量PCR的方法的检测凋亡相关基因Bcl-2、Bax、P53的表达。结果与正常对照相比,急性白血病患者(未缓解组和缓解组)外周血中CD4^+CD25^+Foxp3^+比例明显提高,且治疗缓解后CD4^+CD25^+Foxp3^+表达下调(P<0.05)。凋亡相关基因Bcl-2表达上调,Bax、P53表达下调。结论急性白血病患者外周血CD4^+CD25^+Foxp3^+调节性T细胞明显升高,并且,影响凋亡相关基因Bcl-2、Bax、P53的表达,提示CD4^+CD25^+Foxp3^+调节性T细胞可能通过细胞凋亡信号通路影响急性白血病的发展。

Objective To study the detection of CD4~＋CD25~＋Foxp3~＋ regulatory T cells in cute leukemia patients’ peripheral blood,and discuss its influence on cell apoptosis. Methods Sixty-five cases of patients with acute leukemia（including 33 cases of acute lymphocytic leukemia and 32 cases of acute myeloid leukemia） were divided into un-relieved group（28 cases）,completely relieved group（37 cases）. And 25 healthy volunteers were selected as control group. According to whether accompanied with infection,65 cases of patients were also divided into leukemia accompanied with infection group（39 cases） and not accompanied with infection group（26 cases）. Compared to normal control group,FoxP3 expression was detected in the protein and m RNA level using flow cytometry（FCM） and fluorescent quantitative PCR（q PCR） methods. At the same time,apoptosis related genes Bcl-2,Bax,P53 were detected by q PCR. Results Compared with normal controls,peripheral blood CD4~＋CD25~＋Foxp3~＋ proportion increased significantly in the patients with acute leukemia（un-relieved group and completely relieved group）. And CD4~＋CD25~＋Foxp3~＋ expression reduced after therapy（P〈0.05）. As cell apoptosis related genes,Bcl-2 was unregulated,while Bax and P53 were down regulated.Conclusion CD4~＋CD25~＋Foxp3~＋ regulatory T cells are upregulated in acute leukemia patients’ peripheral blood,and cell apoptosis related genes were influenced,indicating that CD4~＋CD25~＋Foxp3~＋ regulatory T cells influence the development of acute leukemia through apoptosis signaling pathways.