摘要
采用超高效液相色谱-串联四极杆飞行时间质谱(UPLC-Q-TOF/MS)技术快速分析鉴别短管兔耳草的化学成分,为其临床应用提供参考依据,试验采用UPLC-Q-TOF-MS/MS仪器,YMC-Triart C_(18)色谱柱(2.1 mm×100 mm,1.9μm),以乙腈-0.2%甲酸水溶液为流动相梯度洗脱;质谱采用电喷雾(ESI)离子源,在负离子模式下采集数据,通过保留时间、精确分子离子峰和二级质谱裂解碎片,并结合参考文献,对短管兔耳草成分进行鉴定,该实验共鉴别出22个化合物,其中黄酮类化合物11个,苯乙醇苷类化合物6个,环烯醚萜类化合物1个,有机酸类化合物4个。UPLC-Q-TOF-MS/MS方法能快速鉴别短管兔耳草中的各类化学成分,方法简单,快速,可为短管兔耳草的临床应用提供物质依据。
The chemical constituents of Lagotis brevituba were rapidly determined and analyzed by using ultra performance liquid chromatography tandem quadrupole time of flight mass spectrometry （UPLC-Q-TOF-MS/MS） method, providing material basis for the clinical application of L. brevituba. The separation was performed on UPLC YMC-Triart C18 （2. 1 mm × 100 mm, 1.9 μm） column, with acetonitrile-water containing 0. 2% formic acid as mobile phase for gradient elution. The flow rate was 0. 4 mL × min-1 gradient elution and column temperature was 40 ℃, the injection volume was 2 μL. ESI ion source was used to ensure the data collected in a negative ion mode. The chemical components of L. brevituba were identified through retention time, exact relative molecular mass, cleavage fragments of MS/MS and reported data. The results showed that a total of 22 compounds were identified, including 11 flavones, 6 phenylethanoid glycosides, 1 iridoid glucosides, and 4 organic acid. The UPLC-Q-TOF-MS/MS method could fast identify the chemical components of L. brevituba, providing valuable information about L. brevituba for its clinical application.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2017年第11期2123-2130,共8页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81660648)
