热稳定型α-半乳糖苷酶的应用及基因工程研究进展

Advances in application and genetic engineering of thermostable α-galactosidase

α-半乳糖苷酶是一种水解酶,可以将食品、饲料中的不良寡糖等抗营养因子水解,改变其营养成分并被动物吸收利用。该酶广泛存在于植物、动物及微生物中,在饲料、食品等工业以及现代医学中都有应用。目前,开发热稳定的α-半乳糖苷酶并利用基因工程方法进行分子改造和外源表达已成为研究热点。本文对近年来热稳定的α-半乳糖苷酶的应用和基因工程研究进展进行综述。

The α-galactosidase, a kind of hydrolase, can hydrolyze antinutritional factors such as undesirable oligosaccharides and changing the nutritious components to be easily absorbed for animals. The galactosidase commonly exists in plants, animals and microorganisms, which is wildly used in the fields of feed and food industry, modern medicine and so on. In recent years, the development of thermostable α-galactosidase and its molecular modification and heterologous expression with genetic engineering method was a hot research area. In this paper, advances in application and genetic engineering of thermostable α-galactosidase were summarized.