乳腺癌干细胞及其分化细胞中雌激素受体、孕激素受体和人类表皮生长因子受体2的表达

Expression of estrogen receptor,progesterone receptor and human epidermal growth factor receptor 2 in breast cancer stem cells and their differentiated cells

目的探讨乳腺癌干细胞及其分化细胞中雌激素受体(ER)、孕激素受体(PR)和人类表皮生长因子受体2(HER-2)的表达及意义。方法选取2016年1月至2016年10月新乡医学院第一附属医院标本室保存的新鲜乳腺癌标本35例,制备单细胞悬液,利用免疫磁珠分选法进行细胞分选,分选出乳腺癌干细胞(CD44^+CD24^-/low细胞)和分化细胞(CD44^-CD24^-细胞),利用Envision二步法检测乳腺癌干细胞及其分化细胞中ER、PR、HER-2的表达。结果同一患者的乳腺癌干细胞与分化细胞的ER、PR、HER-2表达呈现出3种情况:(1)乳腺癌干细胞阴性表达,分化细胞阳性表达;(2)乳腺癌干细胞阳性表达,分化细胞阳性表达;(3)乳腺癌干细胞阴性表达,分化细胞阴性表达。分化细胞中ER、PR及HER-2阳性表达率显著高于乳腺癌干细胞(P<0.05)。乳腺癌干细胞及其分化细胞共有4种表型,分别为SR^+HER-2^-、SR^+HER-2^+、SR-HER-2^+和SR-HER-2^-,其中,SR表示ER和(或)PR。乳腺癌干细胞的SR-HER-2^-表型比例最大,分化细胞的SR^+HER-2^-表型比例最大;分化细胞的SR^+HER-2^-、SR^+HER-2^+表型比例显著高于乳腺癌干细胞(P<0.05),分化细胞的SR-HER-2^-表型比例显著低于乳腺癌干细胞(P<0.05),但分化细胞与乳腺癌干细胞的SR-HER-2^+表型比例比较差异无统计学意义(P>0.05)。结论乳腺癌干细胞中ER、PR、HER-2阳性表达较低,随着乳腺癌干细胞的不断分化,阳性表达逐渐增加。

Objective To investigate the significance of the expression of estrogen receptor （ER）,progesterone receptor （PR） and human epidermal growth factor receptor 2 （HER-2）in breast cancer stem cells and their differentiated cells.Methods Thirty-five fresh breast cancer specimens were selected from January 2016 to October 2016 in the First Affiliated Hospital of Xinxiang Medical University.The tissue specimens were used to make single cell suspensions.The breast cancer stem cells（CD44 ^＋ CD24^-/low cells）and the differentiated cells（CD44-CD24-cells）were sorted by immunomagnetic beads method.The expressions of ER,PR and HER-2 in breast cancer stem cells and their differentiated cells were detected by Envision two-step method.Results The expressions of ER,PR and HER-2 in breast cancer stem cells and their differentiated cells of the same patient showed three cases：（1） negative expression in breast cancer stem cells,and positive expression in differentiated cells;（2） positive expression in breast cancer stem cells and their differentiated cells;（3） negative expression in breast cancer stem cells and their differentiated cells.The positive expression rates of ER,PR and HER-2 in differentiated cells were significantly higher than those in breast cancer stem cells （P 〈 0.05）.There were four phenotypes including SR ＋ HER-2^-,SR＋ HER-2 ^＋,SR-HER-2 ^＋ and SR-HER-2-in breast cancer stem cells and their differentiated cells（SR represents ER and/or PR）.The proportion of SR-HER-2-phenotype was the largest in breast cancer stem cells.The proportion of SR＋ HER-2-phenotype was the largest in the differentiated cells.The proportions of SR^ ＋ HER-2^-and SR ^＋ HER-2 ^＋ phenotypes in the differentiated cells were significantly higher than those in breast cancer stem cells （P 〈 0.05）.The proportion of SR-HER-2-phenotype in the differentiated cells was significantly lower than that in breast cancer stem cells（P 〈 0.05）.There was no significant difference in the proportion of SR-HER-2 ＋ phenotype between breast cancer stem cells and their differentiated cells （P 〉 0.05）.Conclusion The positive expression of ER,PR and HER-2 was lower in breast cancer stem cells.With the differentiation of breast cancer stem cells,the positive expression of ER,PR and HER-2 increase gradually.