南繁区稻瘟病菌无毒基因的检测

Detection of avirulence genes in Magnaporthe oryzae from South China Crop Breeding Area

为明确南繁区稻瘟病菌(Magnaporthe oryzae)无毒基因的分布情况,对6个无毒基因(ACEI、Avr-Pia、Avr-Pik、Avr-Pita、Avr-Piz-t和PWL2)在南繁核心区和非核心区60个稻瘟病菌菌株中的分布情况进行PCR检测。结果发现,这6个无毒基因在核心区和非核心区的检出率分别为100.00%和96.67%、33.33%和0.00%、100.00%和100.00%、96.67%和90.00%、73.33%和100.00%以及100.00%和73.33%。该结果说明除了无毒基因Avr-Pia在南繁非核心区的稻瘟病菌中未检测到外,其余无毒基因在2个区域的稻瘟病菌中均存在,其中无毒基因Avr-Pik在2个区域的稻瘟病菌中分布率最高,均为100%,其他无毒基因在2个区域的稻瘟病菌中表现出一定的分布率差异。

In order to clarify the distribution of the avirulence genes in Magnaporthe oryzae （He- bert） Barr [-anamorph. Pyricularia oryzae Cav.] from South China Crop Breeding Area, 6 avirulence genes including ACEI,Avr-Pia, Avr-Pik, Avr-Pita ,Avr-Piz-t and PWL2 in 60 isolates of M.oryzae in core and non-core regions were detected by using PCR technique.The results showed that the detection rates of the 6 avirulence genes in rice blast pathogen populations in core and non-core regions were 100.00% and 96.67%, 33.33% and 0.00%, 100.00% and 100.00%, 96.67% and 90.00%, 73.33% and 100.00%, 100.00% and 73.33%,respectively.All the avirulence genes existed in M. oryzae in both re- gions except the Avr-Pia gene, which was not detected in the non-core region. Among them, the Avr- Pik gene had the highest distribution rate of 100% in both regions,and differences of distribution rates existed for the other avirulence genes in the rice blast fungus in the two regions.