油菜内生真菌黄柄曲霉CanS-34A对核盘菌的拮抗性

Antagonistic activity of an endophytic fungus Aspergillus flavipes CanS-34A against Sclerotinia sclerotiorumin oilseed rape

以油菜内生真菌黄柄曲霉CanS-34A为研究材料,通过平板对峙培养、离体叶片法及盆栽试验等试验探究CanS-34A的抑菌谱以及对核盘菌的拮抗效果。结果表明:CanS-34A对供试的16种真菌均有抑菌作用,且对核盘菌(Sclerotinia sclerotiorum)、三叶草核盘菌(S.trifoliorum)、小核盘菌(S.minor)的抑菌率显著高于其他供试菌;3种供试的细菌没有被抑制,且均属于G^-。CanS-34A在PDB中发酵6d,发酵滤液pH上升至7.3,抑菌效果达到最高。培养基中2%(V/V)的发酵滤液对核盘菌的抑菌率达60%。在离体及活体油菜叶片表面涂抹CanS-34A发酵滤液均能有效抑制核盘菌的生长,可有效防治油菜菌核病,表明黄柄曲霉CanS-34A可作为油菜菌核病的生防菌株资源。

Using the endophytic fungus Aspergillus flavipes CanS-34A of oilseed rape as research material,the antimicrobial spectrum of CanS-34A and antagonistic effect on Sclerotinia sclerotiorum were studied through dual-culture,and in vitro and in vivo leaf inoculation. The results showed that the strain CanS-34A exhibited inhibitory effect on 16 kinds of pathogens, with the inhibitory effects on S. sclerotiorum, S. minor and S. trifoliorum significantly higher than on other fungi （P^0.05）. But there were 3 G bacterial strains could not be inhibited by CanS-34A. After CanS-34A was cultured in PDB for 6 d, the antifungal effect reached to the peak and pH increased to about 7.3. Different concentra tions of fermented liquid of CanS-34A have different inhibition effects on S. sclerotiorum and the inhibi- tion rate reached to 60% when 2% fermented liquid was added to PDA media. S. sclerotiorum was inhibited when smeared fermentation broth on leaves in vivo or in vitro. Thus,our results indicated that the strain CanS-34A can effectively inhibit growth of S. sclerotiorum and can be used as a biocontrol agent for controlling stem rot caused by S. sclerotiorum.