鼠肝缺血再灌注后肝组织损伤和胆汁淤积改变

LIVER TISSUE INJURY AND BILE SILT AFTER HEPATIC ISCHEMIA-REPERFUSION IN THE RET

目的:通过病理学检测、肿瘤坏死因子含量检测和统计胆汁生成率来评价鼠肝缺血再灌注后肝细胞损伤及胆汁淤积改变的情况。方法:实验分为假手术组(A组),70%的鼠肝缺血35min再灌注(B组),研究时点为再灌注后的1h、6h、1d、3d、5d。HE染色分析肝组织病理改变,并检测肿瘤坏死因子含量的变化。计算胆汁生成率,常规生化方法检测胆汁、血浆中胆红素含量的变化。结果:70%鼠肝缺血35min再灌注模型炎症反应轻,无肝细胞坏死的发生。与A组比较,B组再灌注后的1h^3d,胆汁生成率及胆汁中结合胆红素含量明显降低;再灌注后的1h^5d,血浆中胆红素含量显著增加。结论:本研究结果表明肝缺血再灌注后,肝脏组织会有炎症反应和胆汁淤积的改变,这为进一步探讨其发生机制提供了理论依据。

Objective：To explore the changs of liver cell injure and bile silt after hepatic ischemia-reperfusion injury in the rat by the detection of pathology, TNF-α and the bile generation rate. Methods：104SD rats were randomly divided into 2 groups：Group A（sham operation group）and Group B （ undergoing 70% hepatic ischemia-35 min-reperfusion ） . Then the rats were subdivided into subgroups according the different time points（1and 6 hours,and1,3,5days after reperfusion）. Tissues of the left liver lobe were collected to undergo microscopy with hematoxilin and eosin staining. The changs of TNF-α was detected. The Bile of the ischemic hepatic lobes and blood form the vena cava were collected to examine the conjugated bilirubin（ CB） in the bile and plasma,and total bilirubin（ TB） and alanine aminotransferase（ALT） in plasma,and the bile generation rate was calculated. Results：Pathological examination showed that there was only a mild inflammation in the liver tissues that had undergone ischemia -reperfusion, and no necrosis of hepatocytes was seen. 1h - 3d and after reperfusion,the bile generation rate and CB level in bile were significant decreased,and the serum TB and CB levels were significantly increased 1 h -5d after reperfusion. Conclusion：The study showed there was inflammation and bile silt in the liver tissues after hepatic ischemia-reperfusion injury in the rat. It provides the theory basis for further exploring it＇s mechanism.