NSE、S-100β和COX-2在1-溴丙烷大鼠亚急性神经损伤中的变化

STUDY ON CHANGES OF NSE,S-100Β AND COX-2 IN 1-BROMOPROPANE-INDUCED SUBACUTE NEUROTOXICITY IN RATS

目的探讨神经元特异性烯醇化酶(Neuron Specific Enolase,简称NSE)、星形胶质源性蛋白(S-100βprotein,简称S-100β)和环氧化酶-2(Cyclooxygenase-2,简称COX-2)在1-溴丙烷大鼠亚急性神经损伤中的变化。方法选取健康雄性Wistar大鼠36只,随机分为0ppm(对照组)和1000ppm1-BP染毒组,每组18只,采用口鼻吸入染毒方法,每天染毒6h连续染毒21d。染毒期间隔天称量并记录动物体重,于染毒后第七、十四和二十一天分批次各处死6只动物,采集脑组织和血液(分离血清),每组2只动物分离大脑、垂体、脊髓、坐骨神经和胫腓神经用于病理检测。用ELISA试剂盒检测大鼠脑组织和血清中NSE,S-100β,COX-2等指标的变化。结果与对照组相比,1000ppm染毒21d的染毒组动物体重增长明显减慢(P<0.05),病理检查可见小脑局部浦肯野细胞萎缩、腰髓灰质空泡变性、胫腓神经部分神经纤维肿胀增粗;在染毒的第一周和第三周,大鼠脑组织中NSE、S-100β和COX-2等神经损伤特异性指标与对照组相比显著增高(P<0.05),血清中NSE在第三周有升高趋势,但与对照组相比差异不显著;S-100β在第三周显著增高(P<0.05);而COX-2在前2周显著增高(P<0.05),第三周与对照组差异不显著;脑组织和血清中S-100β的变化显著相关。结论综合实验动物脑组织和血清中神经特异性损伤指标(NSE、S-100β和COX-2)的变化以及病理检查结果,1-BP大鼠染毒模型可以在现有的试验条件和染毒条件下,引起实验动物的亚急性神经损伤。

Objective To explore the changes of Neuron Specific Enolase, S- 100β protein and Cyclooxygenase - 2 in 1 - bro- mopropane - induced subacute neurotoxicity in rats. Methods Thirty - six Wistar male rats were randomly divided into the Oppm （ control group） and the 1000ppm 1- BP treatment group, 18 rats in each group. The two groups of rats were exposed 1- BP in inhalation with nose - only exposure system for 6h/d, for 21 days.Rats were weighed and recorded every other day after the start of the experiment.After exposure to 1 - BP for 7,14 and 21 days, six rats were sacrificed at each time point, collected the brain tissue and blood （separating serum） and collected urine with metabolism cage on the day before death.Two of six rats were chosen to separate the brain,hypophysis, spinal cord,ischiadic nerve and tibiofibular nerve tissues for histopathological examination.Changes of NSE,S- 100β and COX- 2 in the rat brain tissues and serum were measured by ELISA kits.Results After 21 days exposure, the weight of rats treated with 1000ppm 1 - BP was decl：eased significantly compared with the control group （P〈 0.05）.The main adverse effect in the histopathological examination included cerebellar local Purkinje cell atrophy,lumbar gray matter vacuolar degeneration,tibiofibular nerve fibers swelling and thickening.After exposure to 1- BP for 7 and 21 days,NSE, S- 100β and COX- 2 were increased significantly compared with the control group in the brain of the rats （P 〈0.05）.NSE was increased in the serum on the 21th days,but the difference was not significant compared with the control group.S- 100β was increased significantly in the serum on the 21th days （P〈0.05）.COX- 2 was increased significantly in the serum on the 7th and 14th days, but the difference was not significant compared with the control group on the 21th days.The S- 100β was significantly correlated in brain tissue and serum.Conclusion Comprehensive analysis the results of NSE,S- 100β and COX- 2 in the brain and serum of rats. and the results of histopathological examination,it can be preliminary determined that 1- BP can cause subacute nerve injury in the experimental rats.