DMSO胁迫提高发光细菌毒性实验敏感度的研究

Enhancement of Luminescent Bacteria Sensitivity on Toxic Substances by DMSO

利用二甲基亚砜(DMSO)胁迫,以发光细菌青海弧菌Q67种为实验菌种,通过与标准物质作用测定相对发光值确定其生物毒性效价,对多种食品中可能存在的污染物进行毒性对比研究。结果表明,DMSO浓度在0.1%~1.0%之间不影响发光细菌正常生理反应,同时可以有效的提高其对毒性物质作用毒性。DMSO能明显提高发光细菌的检测敏感度,有效降低半数致死量EC50。对受试重金属汞、铜、镉和锌,其提高效率为43.28%~58.50%;四环类抗生素中盐酸金霉素、盐酸四环素和土霉素的提高效率在8.2%~11.6%之间;抗菌类农药二氰蒽醌、溴菌腈、甲霜灵和异菌氟腚胺的提高效率为10.49%~38.32%。DMSO的胁迫促进效果适用于多种毒性物质,并且能够在短时间内提高作用效果(30 min以内),为食品领域应用生物毒性检测方面提供了一种新的分析方法。

The luminescent bacteria V.qinghaiensis Q67 was selected as test bacterium. Through testing with the standard material, RLU（Relative light unit ） was determined as the biological toxicity titer. The experiment had detected the toxicity of several kinds of toxic substances in food. Results showed that when the concentration of DMSO ranges from 0.1% to 1.0%, DMSO significantly improved the toxicity of Eqinghaiensis Q67 to toxic substance and had little influence to luminescent bacteria. The toxicity test with different concentrations of DMSO is significantly increased and the EC50（concentration for 50% of maximal effect） were reduced observably. The EC50 of copper, mercury, cadmium and zinc were enhanced from 43.28% to 58.50%. The efficiencies of DMSO to Tetracyclines antibiotic including chlortetracycline,tetracycline and oxytetracycline were enhanced from 8% to 11.6%.The toxicity of four antimicrobial pesticides including Two-anthraquinone, Bromothalonil, metalaxyl and iprodine like amine fluoride were promoted from 10.49% to 38.32% .DMSO stress method was suitable for a variety of toxic substances, and also could appeared in a short time （less than 30 min）, which provided a new analytical method for the application of biological toxicity test in in the field of food.