海南龙血树快繁技术研究

Tissue culture and fast propagation in Dracaena cambodiana

以海南龙血树优良母株顶芽和茎段为外植体,切成约2~3 cm厚的段状,经消毒处理后顶端朝下接入MS基本培养基中,2~3 d后待残留在叶柄缝隙中的升汞完全流出后,再接种到MS基本培养基中暗培养,使腋芽长至高约1-1.5 cm时把腋芽从母体上挑下,去顶至0.5~0.8 cm左右接种到MS+6-BA 3.0 mg/L+NAA 0.2 mg/L的培养基中,待芽基部膨大至直径约1.0~1.5 cm大小的球茎(形成愈伤组织)且围绕基部长出一圈高约0.2 cm的小芽时把球茎对剖。接种到MS+6-BA 1.0~2.0 mg/L+NAA 0.2 mg/L的培养基中扩繁,待小芽长到1 cm高时把原团块按2~4个芽/团块分切,增殖倍数可达2.5~3.0倍。几个周期后库存达到一定数量,将团块接入MS+6-BA 0.5 mg/L+NAA 0.1 mg/L的培养基中壮苗长高,经过一段时间的培养后切取H>4.0 cm的单株接种到1/2 MS+IAA 0.3 mg/L的培养基中诱导生根,15~20 d生根率达95%以上。炼苗5 d左右后移栽,成活率达95%以上。

Excellent materials of Dracaena cambodiana top bud and stem were cut into about 2 ~3 cm thick segment. After disinfection treatment,the top was down into the MS basic medium,after 2 ~3 d to be remaining in a crack in the petiole of mercuric chloride flow out completely,and then inoculated into MS as basic culture medium dark culture,the outgrowth of axillary buds supreme approximately 1~1.5 cm the axillary buds from parent to pick,top to 0.5 ~0.8 cm about inoculation to the MS＋6-BA 3.0 mg/L＋NAA 0.2 mg/L medium,bud base expanded to a diameter of about 1.0 ~1.5 cm size corms（callus formation） and around minister out of the circle of high about 0.2 cm bud when the bulb on the profile. Inoculation to the propagation of MS＋6-BA 1.0~2.0 mg/L＋NAA 0.2 mg/L culture medium. When the shoots grew to 1cm the original mass by2~4 bud clumps per cutting,proliferation rate would be up to 2.5~3.0 times. After several cycles of stocks reached a certain number,the training base of mass access MS ＋6-BA 0.5 mg/L ＋NAA 0.1 mg/L sound seedling grow taller,after a period of time of culture cut from H 〉 training base of 4.0 cm plant inoculation to 1/2 MS＋IAA 0.3 mg/L rooting,15~20 rooting rate of more than 95%. The survival rate of the seedlings after transplanting was more than 95%.