非小细胞肺癌p16基因甲基化与血液微转移的关系

Relationship Between p16 Gene Methylation and Hematogenous Micrometastasis in Non-small Cell Lung Cancer

目的探索非小细胞肺癌p16基因甲基化状态及其与血液微转移的相关性。方法采用甲基化特异性PCR(MSP)分析60份Ⅰ期非小细胞肺癌组织和60例良性肺部疾病患者组织中p16基因的甲基化状态,使用逆转录PCR方法检测非小细胞肺癌患者外周血微转移情况。结果 p16基因甲基化在非小细胞肺癌组织与良性病变组织中的检出率分别为46.67%和11.67%,在非小细胞肺癌有血液微转移及无血液微转移患者中的检出率分别为62.96%和33.33%;肺癌组织中p16基因甲基化检出率高于良性病变肺组织(P<0.05),肺癌患者有血液微转移中p16基因甲基化检出率高于无血液微转移患者(P<0.05);不同性别、年龄、病程、肿瘤大小和组织类型的非小细胞肺癌患者p16基因甲基化检出率差异无统计学意义(P>0.05)。结论非小细胞肺癌p16基因的甲基化率较高,与血液微转移密切相关,可能参与肺癌发生和浸润侵袭。

Objective To explore the methylation status of p16 gene in non-small cell lung cancer and its correlation with blood micrometastasis.Methods Methylation-specific PCR （MSP） was used to analyze the methylation status of p16 gene in 60 patients with stage Ⅰ non-small cell lung cancer （NSCLC） and 60 patients with benign lung disease.Reverse transcription-polymerase chain reaction （RT-PCR） was used to detect peripheral blood micrometastasis of non-small cell lung cancer patients.Results The detection rate of p16 gene methylation in non-small cell lung cancer tissues and benign lesions were 46.67% and 11.67%,respectively.The detection rate of p16 gene methylation in non-small cell lung cancer with hematogenous micrometastasis and without hematogenous micrometastasis were 62.96% and 33.33%,respectively.The detection rate of p16 gene methylation in lung cancer tissues was higher than that in benign lung tissues （P 〈0.05）.The detection rate of p16 gene methylation in lung cancer patients with blood micrometastasis was higher than that without blood micrometastasis（P〈0.05）.There was no significant difference in the detection rate of p16 gene methylation in non-small cell lung cancer patients with different genders,ages,courses of disease,tumor sizes and histological types （P〉 0.05）.Conclusion The methylation rate of p16 gene in non-small cell lung cancer is higher,which is closely related to blood micrometastasis and may be involved in the occurrence and invasion of lung cancer.