摘要
以树莓品种"哈瑞太兹"和"红宝玉"1年生枝条带腋芽的茎段为外植体,研究光照时长、植物生长调节剂对腋芽增殖的影响;探讨组培苗生根的最佳培养基,以建立树莓组织培养快繁体系。结果表明:"哈瑞太兹"的培养基为MS+2mg/L 6-BA+0.2mg/L NAA+30g/L蔗糖+8g/L琼脂;"红宝玉"的培养基为MS+2mg/L 6-BA+0.3mg/L NAA+30g/L蔗糖+8g/L琼脂,光照时长12~14h/d最佳;组培苗最佳生根培养基均为1/2MS+1mg/L IBA+2mg/L NAA+30g/L蔗糖+8g/L。
This experiment with raspberry varieties ＂Heritage＂ and＂Hongbaoyu＂annual branches stem segment with axillary bud as explants,Study the illumination time.The influence of plant growth regulator on axillary bud germi- nation;The best medium somaclone take root,To establish a raspberry tissue culture system of rapid propagation. The results show that：＂Haritage＂ culture medium for MS ＋ 2 mg/L 6-BA＋0.2 mg/LNAA 30 g/L ＋ sugar ＋ 8 g/I, AGAR;＂Hongbaoyu＂ medium for MS ＋ 2 mg/L 6-BA ＋0.3 mg/LNAA 30 g/L sugar ＋ 8 g/L AGAR. The best illumination time 12 -14 hours/day; Somaclone best rooting medium are 1/2MS＋1mg/LIBA ＋ 2mg/LNAA＋ 30g/L sugar ＋8 g/L AGAR.
出处
《中国林副特产》
2017年第3期8-10,共3页
Forest By-product and Speciality in China
关键词
树莓
培养基
生根
快繁
Tree blackberry
Culture medium
Take root
Rapid propagation
