摘要
运用酯酶同工酶酶谱分析和ISSR分子标记技术对3株西藏野生黑木耳亲缘性和遗传多样性进行分析。运用丙烯酰胺蛋白电泳技术对野生黑木耳中酯酶同工酶进行电泳;运用PCR-ISSR分子标记技术对野生黑木耳DNA进行随机扩增,扩增产物进行琼脂糖电泳。用Ntsys 2.10e和PopGen32软件对电泳结果进行聚类和遗传分析。结果表明:3株野生黑木耳中察隅和鲁郎地区样品的亲缘性关系较近,样品间遗传分化指数高。结论:本实验为西藏地区野生黑木耳品种分类、资源保护和新品种培育提供了初步依据。
Analysis genetic diversity and relationship of black fungus from different regions of Tibet by method of es- terase isozyme enzyme spectrum and ISSR molecular markers. Using acrylamide protein electrophoresis technology on esterase isozyme of wild black fungus for electrophoresis; take a random amplificatioon of wild fungus by PCR- ISSR molecular marker techonlogy. Conduct product electrophoresis in agarose gel. Clustering and inheritance were analyzed by Ntsys 2.10 and PopGen32 software. Result shows： Samples of Lulang and Chayu have a closer relation- ship among wild fungus, there is a high genetic differentiation index among samples. This study provides a prelimi- nary basis for species classification and hybrid of wild fungus of Tibet.
出处
《中国林副特产》
2017年第3期11-14,共4页
Forest By-product and Speciality in China
基金
西藏自治区自然基金资助项目
