槲皮素对宫颈癌细胞SiHa增殖的影响

Effect of Quercetin on Proliferation of Cervical Cancer Cell SiHa

目的:探讨槲皮素对于人宫颈癌细胞Si Ha的作用及机制。方法:用不同浓度的槲皮素(0、20、40和80μmol/L)处理Si Ha细胞不同时间后(24 h,48 h和72 h),通过噻唑蓝(MTT)法检测细胞活力的变化;通过流式细胞仪检测槲皮素对细胞周期的影响;通过逆转录聚合酶链反应(RT-PCR)和蛋白质印迹法(Western blotting)检测槲皮素对细胞周期蛋白D1(Cyclin D1)表达的影响。结果:MTT法检测结果显示,槲皮素处理Si Ha细胞24 h和48 h后,40μmol/L和80μmol/L组的细胞活力低于对照组(P<0.05);槲皮素处理Si Ha细胞72 h后,20μmol/L、40μmol/L和80μmol/L组的细胞活力低于对照组(P<0.05);在40μmol/L和80μmol/L组,随着培养时间延长,细胞活力较24 h时降低(P<0.05)。流式细胞仪检测结果显示,Si Ha细胞经过20、40和80μmol/L浓度的槲皮素分别处理48 h后,处于G0/G1期的细胞百分比明显高于对照组。RT-PCR和Western blotting结果显示,槲皮素可以下调Si Ha细胞中Cyclin D1蛋白的表达。结论:槲皮素通过下调Si Ha细胞中Cyclin D1蛋白的表达,可以抑制Si Ha细胞的活力和增殖。

Objective To investigate the effect and mechanism of quercetin on human cervical cancer cell line SiHa. Methods： MTT assay was used to detect the effect of quercetin on proliferation of cervical cancer cell SiHa at different times（ 24 h, 48 h and 72 h ） and different concentrations （0, 20, 40 and 80 μmol/L） ; flow cytometry was used to detect the cell cycle in quercetin treated SiHa cells; RT-PCR and Western blotting was used to detect the expression of Cyclin D1 in quercetin treated SiHa cells. Results： The cell viability of SiHa cells by treating with quercetin for 24 h and 48 h in 40 μmol/L and 80 pmol/L group were significantly lower than control group; The cell viability of SiHa cells by treating with quercetin for 72 h in 20 μmol/L, 40 μmol/L 和 80 μmol/L group were significantly lower than control group; the number of G0 /G1 cells in 20 pmol/L, 40 μmol/L and 80 μmol/L group was significantly more than control group by treating with quercetin for 48 h; the protein level of Cyclin D1 was down-regulated in quercetin treated group. Conclusions： Quercetin could inhibit cell vitality and cell proliferation by down-regulating the expression of Cyclin D1 in cervical cancer cell SiHa.