超声波辅助纤维素酶提取扫帚菜多糖及其抗氧化研究

Optimization of Ultrasonic-assisted Cellulase Extraction and Antioxidant Activity on Polysaccharides from Kochia Scoparia(Linn.) Schrad

【目的】通过响应面法优化超声辅助纤维素酶提取扫帚菜多糖的工艺,评价多糖的抗氧化活性。【方法】在单因素试验的基础上,以多糖提取率为响应值,选择酶用量、超声温度、超声时间和液料比进行Box-Behnken试验设计,研究了各工艺条件及其交互作用对扫帚菜多糖提取率的影响,得到了二次多项式回归方程模型,并以猪油的抗氧化性能来评价多糖的抗氧化活性。【结果】扫帚菜多糖的最佳提取工艺条件为:酶用量2.1%、超声温度51℃、超声时间20 min、液料比25 m L/g,该条件下扫帚菜多糖的提取率为65.93 mg/g,与理论预测值的相对误差为0.21%。扫帚菜多糖对猪油有一定的抗氧化能力。【结论】利用响应面法优化超声辅助纤维素酶提取扫帚菜多糖的工艺有效、可靠,扫帚菜多糖能有效提高猪油产品的货架期。

【Objective】The aim of the study was to optimize the ultrasonic-assisted cellulase extracting technology of polysaccharides from Kochia Scoparia(Linn.)Schrad by response surface methodology and to evaluate the antioxidative activity of polysaccharides.【Method】Based on a single factor experiment,a quadratic polynomial regression model was established about the dependent variable(extraction yield of polysaccharide)and independent variables(cellulase amount,ultrasonic temperature,ultrasonic time and liquid-material ratio),using Box-Benhnken design and response surface methodology. The antioxidant properties of lard were used to evaluate the antioxidative activity of polysaccharides.【Results】The optimum process parameters of ultrasonic-assisted cellulase extraction of polysaccharides from Kochia Scoparia(Linn.)Schrad were shown as followed,cellulase amount 2.1%,ultrasonic temperature 51℃,ultrasonic time 20 min and liquid-material ratio 25 m L/g. Under the optimal process conditions,the yield of polysaccharide was 65.93 mg/g and the relative error was 0.21% compared to the predictive value,indicating that the feasible model fitted well with the experimental data. The polysaccharides had a certain antioxidant capacity on the lard. 【Conclusion】The ultrasonic-assisted cellulase extracting technology of polysaccharides from Kochia Scoparia(Linn.)Schrad by response surface methodology was effective and reliable. The polysaccharides could effectively improve the shelf life of lard.