火绒草的咖啡酰类成分研究

Caffeic Acid Isolated from Leontopodium leontopodioides

目的:研究火绒草的化学成分。方法:采用硅胶、葡聚糖凝胶、大孔树脂、制备液相等色谱技术进行化学成分分离,依据理化性质和波谱数据鉴定化合物结构。结果:从火绒草正丁醇萃取部位分离出11个化合物,分别鉴定为:绿原酸甲酯(1)、4-O-咖啡酰奎宁酸甲酯(2)、5-O-咖啡酰奎宁酸甲酯(3)、绿原酸(4)、4-O-咖啡酰奎宁酸(5)、1,3-O-二-咖啡酰奎宁酸(6)、3,4-O-二-咖啡酰奎宁酸甲酯(7)、4,5-O-二-咖啡酰奎宁酸甲酯(8)、3,5-O-二-咖啡酰奎宁酸(9)、3,4-O-二-咖啡酰奎宁酸(10)、4,5-O-二-咖啡酰奎宁酸(11)。结论:其中,化合物1~9为首次从该植物中分离得到。

Objective ：To study the chemical constituents of Leontopodium leontopodioides. Methods：The compounds were isolated by column chromatography of silica gel, MCI, Sephadex LH-20 and HPLC from the n-butyl alcohol extract of Leontopodium leontopo- dioides,their structures were elucidated by NMR, MS spectra. Results ： Eleven compounds were isolated from Leontopodium leontopo- dioides and identified as methyl chorogenate（ 1 ） ,4-O-caffeoylquinic acid methyl ester（ 2 ） ,5-O-caffeoylquinic acid methyl ester（ 3 ）, chorogenaic acid（g）,4-O-caffeoylquinic acid（5）,1,3-di-O-caffeoylquinic acid（6）,3,g-di-O-caffeoylquinic acid methyl ester（7）,4, 5-di-O-caffeoylquinic acid methyl ester （ 8 ）, 3,5-di-O-caffeoylquinic acid （ 9 ）, 3, g-di-O-caffeoylquinic acid （ 10 ）, 4,5-di-O-caf- feoylquinic acid（ 11 ）. Conclusion：Compounds 1 -9 are isolated from this plant for the first time.