润燥灵对干燥综合征模型小鼠Th17/Treg细胞的影响

Effect of Runzaoling on Th17/Treg cells of mouse with Sjogren's Syndrome

目的:探讨润燥灵对免疫诱导性干燥综合征(SS)模型小鼠外周血辅助性T细胞17(Th17)和调节性T细胞(Treg)的影响。方法:将90只C57bl/6j小鼠随机分成为正常组、模型组、润燥灵36g/kg组、润燥灵18g/kg组、润燥灵9g/kg组和泼尼松0.2g/kg组,每组15只,除正常组外,其余各小鼠采用免疫诱导法建立SS模型,造模第5 w开始灌胃给药,每周测量小鼠饮水量,给药4 w后眼球取血,断颈处死,分离颌下腺,HE梁色后病理检测,PCR检测颌下视黄醇类核内受体(RORγt)和转录因子叉头框蛋白3(Fox P3)的表达,流式细胞术检测Thl7细胞和CD4^+CD25^+T细胞的含量。结果:与模型组比较,润燥灵36g/kg组和润燥灵18g/kg组小鼠饮水量增加,颌下腺淋巴细胞浸润减少。PCR法结果显示,与正常组比较,模型组RORγt m RNA和Fox P3 m RNA表达量升高;与模型组比较,润燥灵36gkg、润燥灵18g/kg和泼尼松0.2g/kg组RORγt m RNA和Fox P3 m RNA表达量降低。流式细胞术结果显示,与模型组比较,润燥灵36gkg组和泼尼松0.2g/kg组CD4^+/CD4^+CD25^+T比值降低。结论:润燥灵能够抑制SS模型小鼠RORγt m RNA和Fox P3 m RNA表达量,调节Thl7/Treg细胞平衡作用。

Objective：To explore the effect of Chinese medicine Runzaoling on peripheral blood helper T ceils 17 （Th17）and regulatory T cells （Treg） of mouse with sjogreng syndrome. Methods：90 C57bl/6j mice were randomly divided into normal group, model group, Runzaoling 36g/kg, 18g/kg,9g/kg group and prednisone group 0.2g/kg, 15 rats in each group. Except the normal group, other mice were established SS model After 5 weeks＇administration, the amount of drinking water was measured, once a week, 9 weeks＇administration later, mice were sac- rificed, the blood and submandibular gland were collected, submandibular glands were detected by HE staining. Expressions of submandibu- lar of retinoid receptor （RORγt） and the transcription factor forkhead box protein 3 （ FoxP3 ） were detected by PCR. The contents of Thl7 cells and CD25^＋T CD4^＋ cells were detected by Flow eytometry. Results：Compared with the model group, drinking water increased and lym- phocyte infiltration of submandibular gland reduced in Runzaoling 36g/kg and 18g/kg group. PCR results showed that, compared with the normal group, the expressions of RORs, t and FoxP3 mRNA increased in the model group. Compared with the model group, expressions of RORγt and FoxP3 mRNA decreased in Runzaoling 36gkg, runzaoling 18g/kg and prednisone group 0.2g/kg. Flow cytometry results showed that, compared with the model group, CD4 ^＋/CD4^＋ CD25^＋ T decreased in runzaoling 36gkg group and prednisone group 0.2g/kg. Conclu- sion：Runzaoling could inhibit the expressions of RORγt mRNA and FoxP3 mRNA in SS mouse, and regulate Thl7/Treg cells.