实时荧光定量检测盐胁迫下香蕉幼苗CaM和Ca^2＋-ATPase基因的相对表达量

The Relative Expression of CaM and Ca^(2+)-ATPase Genes in Banana Seedlings under Salt Stress by Real-time Fluorescence Quantitative Assay

通过增加外源CaCl_2和钙离子螯合剂EGTA处理后,研究香蕉幼苗在60 mmol/L NaCl人工模拟的盐胁迫0、4 h、8 h、12 h、24 h和48 h不同时间下,测定其叶片和根的CaM和Ca^(2+)-ATPase基因的相对表达量。结果表明,在NaCl胁迫过程中,香蕉幼苗在正常的生长条件下,其根和叶片的CaM基因和Ca^(2+)-ATPase基因均有表达。巴西蕉幼苗根在盐胁迫过程中Ca M基因没有发生显著变化,但是粉蕉根却发生了显著变化,这可能与粉蕉耐盐性高于巴西有关。随着盐胁迫时间的延长,巴西蕉和粉蕉幼苗根的Ca^(2+)-ATPase基因的相对表达量均呈现先上升后下降的趋势。

The experiments were conducted artificial simulated salt stress environment at 60 mmo/L NaC1, Banana seedlings under NaC1 stress 0, 4 h, 8 h, 12 h, 24 h and 48 h at different times, by adding exogenous CaC12 and calcium chelator EGTA treatment, measured CaM gene and Ca2＋-A TPase gene content on roots and leaves. The results showed that, the CaM gene and Ca2＋-A TPase gene of banana seedlings under the normal growth condition were all expressed in roots and leaves under NaCl stress. The CaM gene was no significant change in the roots of Brazil banana seedlings under salt stress, but has changed dramatically in the Fenjiao banana,which may be related to the higher salt tolerance of Fenjiao than Brazil banana.The relative expression of Ca2＋-A TPase gene in the root of Brazil and Fenjiao banana showed a tendency to increase first and then decrease with the prolongation of salt stress time.