荷兰菊不同外植体组织培养快繁体系

Tissue Culture and Regenerated of the Different Explants of Aster novibelgii

本试验分别以荷兰菊茎段、花托、叶片为外植体,通过不同的处理方式,建立其高频再生组培快繁体系。结果表明:茎段诱导丛生芽最佳培养基为MS+6-BA 1.0 mg/L+NAA 0.3 mg/L+GA30.5 mg/L,平均诱导不定芽数为4.95个;花托诱导不定芽最佳培养基为MS+6-BA 1.5 mg/L+NAA 0.6 mg/L,诱导率最高为75%;叶片诱导不定芽最佳诱导培养基为MS+6-BA 2.0 mg/L+NAA 1.0 mg/L,诱导率67.4%;最佳增殖培养基为MS+6-BA 1.0 mg/L+NAA 0.4 mg/L,其增殖倍数为8.3倍,且幼苗生长情况优于其它处理;最佳生根培养基为1/2 MS+NAA 0.4 mg/L。该研究建立了荷兰菊稳定高效的组培再生体系,为工厂化育苗与大面积推广奠定了基础。

The stems, receptacle and leaf of Aster novi-belgii as explants respectively were studied for high frequency and rapid propagation through tissue culture by different ways. The results indicated that the best culture medium for inducing cluster buds from stem segments was MS＋6-BA 1.0 mg/L＋NAA 0.3 mg/L＋GA3 0.5 mg/L, and the average adventitious receptacle was 4.95; The best culture medium for inducing adventitious buds from receptacle was MS＋6-BA 1.5 mg/L＋NAA 0.6 mg/L, the highest induction rate was 75%; The best culture medium for inducing adventitious buds from leaf was MS ＋6-BA 2.0 mg/L ＋NAA 1.0 mg/L, the induction rate arrived 67.4%; The optimal proliferation medium was MS＋6-BA 1.0 mg/L＋NAA 0.4 mg/L, and the multiplication coefficient was 8.3, the seedling growth was better than other treatments; The best rooting medium was 1/2MS＋NAA 0.5 mg/L. The study not only established a stable and efficient regeneration system of tissue culture and pigment, but also laid the foundation for the promotion factory seedling and large area.