电针对偏头痛超早期大鼠下行通路5-HT_(1A)受体表达作用机制研究

Study on mechanism of electro-acupuncture on 5-HT_(1A) receptor expression in descending pain pathway of migraine rats in ultra-early stage

目的研究电针对偏头痛超早期大鼠下行通路中5-HT_(1A)受体的调控作用。方法将成年雄性SD大鼠50只随机分为对照组(C组)、模型组(M组)、单穴组(EA1组)、配穴组(EA2组)、非穴组(NA组)5组,C组只予以电极安置手术,其余各组在电刺激后予以相应电针治疗。第0、2、4、6天测量大鼠头面部与后足底的机械痛阈,采用实时荧光定量PCR和免疫荧光对偏头痛超早期大鼠的中缝大核与三叉神经脊束核尾侧亚核的5-HT_(1A)受体表达进行检测。结果在第0天,各组大鼠机械痛阈组间差异无统计学意义(P>0.05);在第2、4、6天,M组大鼠机械痛阈低于C组,差异有统计学意义(P<0.01);EA1组、EA2组分别与C组比较,差异无统计学意义(P>0.05);NA组与M组比较,差异无统计学意义(P>0.05)。M组5-HT_(1A)受体表达与C组、EA1组和EA2组相比升高,差异有统计学意义(P<0.01);与NA组比较,差异无统计学意义(P>0.05)。结论电针对偏头痛超早期大鼠中缝大核与三叉神经脊束核尾侧亚核部位的5-HT_(1A)受体表达能起到抑制作用,可能是针刺治疗偏头痛的机制之一。

Objective To study the effect of electro-acupuncture on expression of 5-HT1A receptor in the descending pain pathway of migraine rats in ultra-early stage. Methods 50 adult male SD rats were randomly divided into five groups ： control group （ group C）, Model group （group M）, electro-acupuncture at GB20 group （ group EA1 ）, electro-acupuncture at GB20 and GB34 group （ group EA2）, none-acupuncture point group （group NA） . M, EA1, EA2 and NA were given electric stimulation to mimic migraine attacks every other day and were treated by electro-acupuncture everyday while C was only given surgery of planting electrodes. On the day 0, 2, 4 and 6, the mechanical pain thresholds of face and hind paw of each rat were tested. The 5-HT1A receptor expression in RMg and TNC were detected using real time PCR and Immunofluorescence. Results On day 0, there were no significant differences in mechanical pain thresholds of the face and hind paw between groups. On the day 2, 4 and 6, mechanical pain thresholds of group M was significantly lower than group C, the difference was statistically significant （ P 〈 0.01 ） ; neither EA1 nor EA2 had a significant difference with group C （P 〉 0.05）, and there was no significant difference between group NA and group M （P 〉 0. 05 ） . According to the results of real time PCR and Immunofluorescence, 5-HT1A receptor expression of group M was significantly higher than group C, group EA1 and group EA2, the differences had statistical significance （P 〈 0. 01 ）; but had no significant significance group NA. Conclusion There might be inhibition on the expression of 5-HT1A receptors in RMg and TNC of the migraine rats by electro- acupuncture in ultra-early stage, and this is probably one of the mechanisms of the anti-migraine effect of electro-acupuncture.