高效液相色谱法检测小麦和土壤中叶菌唑的残留

Determination of metconazole residues in wheat and soil by high performance liquid chromatography

目的建立固相萃取-高效液相色谱法测定土壤、麦粉和小麦植株中叶菌唑残留的方法。方法土壤、麦粉和小麦植株样品采用乙腈提取,经Silica-SPE固相萃取柱净化,Eclicpse Plus C_(18)色谱柱分离,采用高效液相色谱法进行检测,以外标法定量。结果叶菌唑在0.1~10 mg/L范围内呈良好的线性关系,标准曲线方程为Y=37.395X-1.0902,相关系数为0.9999。在加标水平为0.05~2.0 mg/kg范围内,叶菌唑在土壤、麦粉和小麦植株中的平均回收率为87.2%~100.9%,相对标准偏差为0.2%~8.7%。叶菌唑的最低检测浓度为0.05mg/kg。结论该方法简便、快速、准确,可为叶菌唑在小麦中的残留分析提供技术依据。

Objective To establish a method for the determination of metconazole residues in soil, wheat flour and wheat plant by solid phase extraction-high performance liquid chromatography（SPE-HPLC）. Methods The metconazole from soil, wheat flour and wheat plant samples were extracted with acetonitrile, cleaned up by a Silica-solid phase extraction column（Silica-SPE）, separated by an Eclicpse Plus C18 column, and detected by HPLC with the external standard method for quantification. Results Metconazole had a good linear relationship in the range of 0.1 to 10 mg/L. The standard curve equation was Y=37.395X-1.0902 with the correlation coefficient of 0.9999. The average recoveries of metconazole in soil, wheat flour and wheat plant spiked in the range of 0.052.0 mg/kg were 87.2%100.9% with the relative standard deviations（RSDs） of 0.2%8.7%. The limit of quantification was 0.05 mg/kg. Conclusion The method is simple, rapid and accurate, which can provide technical basis for metconazole residue analysis in wheat.