固相微萃取-气质联用法测定2种延命草属植物的挥发性物质

Determination of volatile components from two Plectranthus genera plants by solid-phase microextraction combined with gas chromatography-mass spectrometry

目的考察2种形态上类似的延命草属植物在挥发性物质的组成与相对含量上的不同。方法以Plectranthus tomentosa(碰碰香)和Plectranthus sp.(未定名)为研究对象,采用固相微萃取-气相色谱-质谱联用法(solid-phase microextraction combined with gas chromatography-mass spectrometry,SPME-GC-MS)分析叶片的挥发性成分,再使用峰面积归一化法确定成分的相对含量。结果通过NIST谱库检索,从Plectranthus tomentosa中初步鉴定出高匹配成分39个,占所有检出峰总相对含量的81.78%;从Plectranthus sp.中鉴定高匹配成分41个,占所有检出峰总相对含量的80.62%。挥发性物质中包含3个高含量成分,分别为柠檬烯、乙酸龙脑酯、石竹烯。这2种植物包含25种共有成分,同时其挥发性物质也存在差异,Plectranthus tomentosa中包含13种特异性成分,而Plectranthus sp.中包含16种。结论本研究为2种延命草属植物的进一步开发和利用提供参考。

Objective To investigate the differences of the compositions and relative content of volatile substances between two similar Plectranthus genera plants.Methods Plectranthus tomentosa and Plectranthus sp.were taken as the samples,solid-phase microextraction combined with gas chromatography-mass spectrometry（SPME-GC-MS） was used to analyze the volatile components,and the peak area normalization method was used to determine the relative content of the components.Results Using NIST spectrum library retrieval,39 high matching components were identified from Plectranthus tomentosa,with the relative content of 81.78%,and 41 high matching components were identified from Plectranthus sp.with the relative content of 80.62%.Among the volatile substances,3 high content components were found,which included limonene,bornyl acetate,and caryophyllene.These two plants contained 25 common components,and also Plectranthus tomentosa contained 13 specific components,and Plectranthus sp.contained 16 components.Conclusion This study provides the references for the further development and utilization of these two Plectranthus genera plants.