宫颈癌、癌旁组织酪氨酸激酶受体B、脑源性神经营养因子、血管内皮生长因子-C及血管内皮生长因子受体-3的表达

Expressions of tyrosine kinase receptor B,brain-derived neurotrophic factor,vascular endothelial growth factor-C,and vascular endothelial growth factor receptor-3 in cervical cancer and cervical paracancerous tissues

目的通过研究酪氨酸激酶受体B(TrkB)、脑源性神经营养因子(BDNF)、血管内皮生长因子-C(VEGF-C)及血管内皮生长因子受体-3(VEGFR-3)在宫颈癌、癌旁组织和正常宫颈组织中的表达情况,分析TrkB、BDNF、VEGF-C及VEGFR-3与宫颈癌临床病理特征的关系,探讨宫颈癌及癌旁组织凋亡抑制因子及血管内皮生长因子(VEGF)表达的临床意义。方法选择宫颈浸润癌患者72例,以正常宫颈组织72例为对照,构建包括宫颈癌组织、癌旁组织、正常宫颈组织在内的组织芯片,采用免疫组化法检测TrkB、BDNF、VEGF-C和VEGFR-3的表达,比较TrkB、BDNF、VEGF-C、VEGFR-3在不同组织中表达差异及其临床意义。结果共构建组织芯片6张。TrkB、BDNF、VEGF-C和VEGFR-3在宫颈癌各自的表达率均明显高于癌旁组织,正常宫颈组织中几乎不表达;TrkB、VEGF-C、VEGFR-3在宫颈癌组织中的阳性率与有无脉管侵犯及淋巴结转移有关(P<0.01);癌旁组织中TrkB、BDNF、VEGF-C和VEGFR-3表达除与上述因素有关外,还与肿瘤直径有关(均P<0.05)。结论宫颈癌组织中TrkB、BDNF、VEGF-C和VEGFR-3均有较高表达率,癌旁组织中存在一定量的表达,其表达强度与肿瘤直径、有无脉管浸润及淋巴转移显著相关。TrkB、BDNF、VEGF-C及VEGFR-3表达具有协同性。TrkB、BDNF、VEGF-C及VEGFR-3在宫颈癌及癌旁组织中共同参与了宫颈癌的浸润及淋巴转移,可能作为新的评价宫颈癌预后的生物学指标。

Objective To analyze the relationships between tyrosine kinase receptor B （TrkB）, brain-derived neurotrophic factor （BDNF） , vascular endothelial growth factor-C （VEGF-C） , vascular endothelial growth factor receptor-3 （VEGFR-3） and clinicopathological characteristics of cervical cancer by researching the expressions of TrkB, BDNF, VEGF-C, and VEGFR-3 in cervical cancer, cervical paraeancerous tissues, and normal cervix, explore the clinical significance of apoptosisinhibited molecule and vascular endothelial growth factor （VEGF） expressions in cervical cancer and cervical paracancerous tissues. Methods Seventy-two patients with cervical invasire cancer were selected, and 72 women with normal cervical tissues were selected as control group. Tissue microarray included cervical cancer tissues, cervical paraeancerous tissues, and normal cervical tissues, immunohistochemical method was used to detect the expressions of TrkB, BDNF, VEGF-C, and VEGFR-3, the expression differences of above-mentioned indexes were compared, and the clinical signifi- cance was analyzed. Results Six tissue chips were constructed. The expression rates of TrkB, BDNF, VEGF-C, and VEGFR-3 in cervical cancer were significantly higher than those in cervical paracancerous tissues, and the above-mentioned indexes rarely expressed in normal cervical tissues. The positive rates of TrkB, VEGF-C, and VEGFR-3 in cervical cancer were correlated with vascular invasion and lymph node metastasis （P〈0. 01 ） , The expressions of TrkB, BDNF, VEGF-C, and VEGFR-3 were correlated with vascular invasion, lymph node metastasis, and the diameter of tumor （ all P〈0. 05） . Conclusion The expression rates of TrkB, BDNF, VEGF-C, and VEGFR-3 in cervical cancer are high, and there is a certain amount of expressions of TrkB, BDNF, VEGF-C, and VEGFR-3 in cervical paracancerous tissues, the expression rates is significantly correlated with the diameter of tumor, vascular invasion and lymph node metastasis. The expressions of TrkB, BDNF, VEGF-C, and VEGFR-3 have synergy. TrkB, BDNF, VEGF-C, and VEGFR-3 are involved in invasion and lymph node metastasis of cervical cancer in cervical cancer tissues and cervical paracancerous tissues, which may be used as new biological markers for evaluating the prognosis of cervical cancer.