维多利亚蓝B探针吸收光谱法测定药物中的卡托普利

Determination of captoril in the drug by absorption spectrometry with vitoria blue B as probe

建立了快速、准确测定卡托普利的单波长、双波长吸收光谱法,并探讨了吸收光谱特征和共存物质的影响。在p H为7.93的Tris-盐酸缓冲溶液中,卡托普利与维多利亚蓝B反应生成具有1个明显正吸收峰和1个明显负吸收峰的蓝色离子缔合物,最大正吸收波长位于610 nm,最大负吸收波长位于488 nm,表观摩尔吸光系数分别为2.04×10~4L/(mol·cm)(610 nm)和2.03×10~4L/(mol·cm)(488 nm)。当采用双波长法测定时,表观摩尔吸光系数为4.07×10~4L/(mol·cm),卡托普利的质量浓度在0~3.0 mg/L范围内服从比尔定律。该方法简便、快速,有较高的准确度和选择性,可用于市售卡托普利药物中卡托普利的测定。

A rapid and accurate single wavelength and double wavelength absorption spectrometry for deteimination of captopril is established.The absorption spectral characteristics and the effects of coexisting substances are discussed.In Tris-hydrochloric acid buffer solution of pH 7. 93, captoril reacts with vitoria blue B to form a blue ion association complex with an obvious positive absorption peak and an obvious negative absorption peak, and the maximum positive absorption wavelength locates at 610 nm and the maximum negative absorption wavelength locates at 488 nm. The apparent molar absorptivity （K） of them is 2. 04×10^4L/（mol·cm） （610 nm） and 2. 03×10^4 L/（mol·cm） （488 nm） respectively.When the double wavelength spectrometry is used to determine concentration of captoril, its apparent molar absorptivity （K） is 4. 07×10^4 L/（mol·cm） ,Beer＇ s law is obeyed in definite mass concentration range within 0-3.0 mg/ L of captoril.The method is simple and rapid, and has higher accuracy and selectivity, and it can be used to determine the content of captoril in commercially available captoril drugs.