灵芝孢子油中7个脂肪酸的含量测定

Determination of 7 fatty acids in Ganoderma lucidum spore oil

目的:建立气相色谱法测定灵芝孢子油中7个脂肪酸(肉豆蔻酸、十五烷酸、棕榈酸、十七烷酸、硬脂酸、油酸和亚油酸)含量的分析方法。方法:采用HP-FFAP(30 mm×0.32 mm×250μm)色谱柱,程序升温的方式对灵芝孢子油中7个脂肪酸含量进行测定研究。结果:各脂肪酸线性关系良好,重复性RSD均在3.0%以下,加样回收率(n=9)在96.6%~103.3%之间;样品测定结果显示在灵芝孢子油脂肪酸中,不饱和的脂肪酸约占总量的76.41%,以油酸和亚油酸为主,饱和脂肪酸以棕榈酸和硬脂酸为主,约占总量的18.05%。结论:该方法可作为检测灵芝孢子油中7个脂肪酸的推广方法。

Objective ： To develop a method for the determination of 7 fatty acids （ nutmeg acid, pentadecanoic acid, palmitic acid, heptadecanoic acid, stearic acid, oleic acid, linoleic acid ） in C, anoderma lucidurn spore oil using gas chromatography （ GC ）. Methods： The chromatographic column of HP-FFAP and the method of temperature programmed were adopted for quantitative analysis of 7 fatty acids in Ganoderma lucidum spore oil. Results： The linearity of 7 fatty acids in Ganoderma lucidum spore oil was good, RSD of repeatability was with less than 3.0%, and the average recoveries （ n=9 ） were 96. 6%-103.3%. The results showed that unsaturated fatty acids, rich with oleic acid and linoleic acid, accounted for about 76.41% of the total fatty acids in Ganoderma lucidum spore oil, saturated fatty acids, mainly as palmitic acid and stearic acid, accounted for about 18.05% of the total. Conclusion： The method has been successfully applied in determination of 7 fatty acids in Ganoderma lucidum spore oil.