IL-17、RORγt在百草枯中毒小鼠急性肺损伤中的表达

Roles of interleukin-17 and RORγt in a murine model with paraquat-induced acute lung injury

目的:观察Th17型细胞因子IL-17A和特异性转录因子维甲酸相关孤独受体(RORγt)在百草枯(PQ)中毒小鼠急性肺损伤的表达及意义。方法:雄性昆明小鼠随机分为对照组和模型组,各8只。模型组予一次性腹腔注射20 mg/kg PQ建立染毒模型,对照组予同法注射等量生理盐水,48 h后处死小鼠并取材。HE染色观察肺组织病理变化;原位末端缺刻标记法观察细胞凋亡并计数;酶联免疫吸附法检测血清IL-17A的水平;免疫荧光定量PCR法和蛋白质免疫印迹法检测肺组织RORγt m RNA及IL-17蛋白的表达。结果:与对照组相比,模型组小鼠肺组织炎症及细胞凋亡显著增加;血清IL-17A含量明显增多(P<0.05);肺组织RORγt m RNA和IL-17蛋白的表达均增高。结论:Th17可能通过分泌IL-17参与PQ中毒小鼠急性肺损伤的炎症过程,并在其致病中发挥重要作用。

Objective To explore the roles of Thl7 cells in paraquat-induced acute lung injury （ALI） in a murine models through investigating the expression of Th 17 type cytokine IL-17 and the specific transcription factor of Thl7 cells-RORγt mRNA. Methods Male Kunming mice were randomly divided into the normal control group and the poisoned group. The ALI model induced by PQ poisoning was produced by intraperitoneal injection PQ solu- tion （20 mg/kg ） , and the normal control group was given normal saline solution with the same volume. After treatment for 48 hours, the animals were sacrificed. H＆E staining and TUNEL staining were done to measure histo- logical changes and apoptosis. Serum level of Thl7 related-cytokines was assayed by ELISA. The mRNA expression of RORγt and the protein expression of IL-17 in the lung tissue were detected by real time-PCR assay and western blot, respectively. Results Compared with the normal control group, the lung inflammation and apopto- sis were significantly higher in the poisoned group. The serum level of IL- 17A was significantly increased （P 〈 0.05 ） and the lung parenchyma ROR~/t mRNA and IL-17 protein levels were significantly increased, Conclusion IL-17 plays an important role in the pathogenesis of ALI induced by paraquat poisoning.