HDAC5对胃癌SGC-7901细胞增殖和凋亡的影响

Effects of HDAC5 on the proliferation and apoptosis of the gastric cancer cell line SGC-7901

目的:探讨HDAC5在胃癌细胞中的表达及其对胃癌SGC-7901细胞增殖和凋亡的影响。方法:通过Western blot检测HDAC5和Twist1在胃癌细胞株及正常胃黏膜上皮细胞中的表达。使用MTT及流式细胞术分别检测HDAC5和Twist1对胃癌SGC-7901细胞增殖和凋亡的影响。结果:HDAC5和Twist1在胃癌细胞株中的表达量均明显高于正常胃黏膜上皮细胞(P<0.05)。沉默HDAC5的表达可使胃癌SGC-7901细胞中Twist1表达降低,并抑制其增殖、促进凋亡;而过表达HDAC5作用相反(P<0.05)。此外,沉默Twist1可抑制SGC-7901细胞的增殖、促进其凋亡(P<0.05)。结论:HDAC5可能通过上调Twist1的表达水平促进胃癌细胞的增殖、抑制凋亡,从而促进胃癌的发生发展。

Objective： To investigate HDAC5 expression in gastric cancer cell lines and its effects on the proliferation and apoptosis of the gastric cancer line SGC-7901. Methods： The expression patterns of HDACS and Twist1 in gastric cancer cell lines and normal gastric mucosal cells were detected by Western blot. The effects of HDAC5 and Twist1 on the proliferation and apoptosis of SGC-7901 cells were analyzed by MTT and flow cytometry, respectively. Results： The expression of HDACS and Twist1 in gastric cancer cell lines were significantly higher than that in normal gastric mucosal cells （P〈0.05）. HDAC5 knockdown significantly down-regulated Twist1 expres- sion, inhibited cell proliferation, and induced apoptosis in SGC-7901 cells, whereas HDACS overexpression exhibited an opposite effect （P〈O.05）. Moreover, Twist1 knockdown significantly inhibited cell proliferation and induced apoptosis in SGC-7901 cells （P〈0.05）. Conclusion： HDACS may promote cell proliferation and inhibit apoptosis in gastric cancer cells by upregulating Twist1 expression, thus promoting the initiation and development of gastric cancer.