猪圆环病毒3型检测及其Cap结构序列和抗原性预测分析

Survey on Detection and Analyses of Cap Antigenicity Prediction of Porcine Circovirus Type 3 Isolated from Partial Provinces of Southern China

近期报道在美国多个州的养猪场发现了一种新型的猪圆环病毒3型毒株(porcine circovirus 3,PCV3)。为了确定PCV3是否已经在我国南方部分省份的猪场中广泛存在,并对目前PCV2疫苗是否对PCV3感染具有交叉保护作用作出科学预测,笔者对我国南方几个省份疑似猪皮炎肾病综合征的病猪组织样品进行PCV3巢式PCR检测及其核衣壳蛋白质(capsid protein,Cap)结构的PyMol和抗原表位服务器在线模拟分析。结果首次在这些病料中检测到PCV3的存在,部分病料表现为PCV2和PCV3共感染的特性。对其Cap结构及抗原性分析发现,PCV2和PCV3表现出很大的差异。因此,推测PCV2和PCV3不具有交叉免疫保护的特性,新的疫苗研究和开发,对于将来控制PCV3流行是必要的。

A novel genotype of porcine circovirus type 3 widely distributed in several states in America. In order （PCV3） has recently been found to be to confirm that whether PCV3 has been distributed in southern China, and make prediction about whether the PCV2 vaccines have protec- tive effect on PCV3. In this study, nested-PCR was used for detection of PCV3 samples collected from partial provinces of southern China, PyMol and web server were used to analyze the struc- ture of the PCV3 capsid protein （Cap）. PCV3 was detected in the pigs with PDNS-like syn- drome, and these pigs in farms of several provinces of southern China were co-infected with both PCV 2 and PCV 3. Analyses of the orimarv seouences and the 3-Dimensional （3D） structures of both PCV 2 and PCV 3 Cap demonstrates large, distinct differences in their antigenic epitopes, suggesting cross-protection between PCV2 and PCV3 is impossible. Therefore, the development of novel vaccines will most likely be required to control PCV3 prevalence and infections on farms.