爆米花型金纳米用于信号放大构建夹心型免疫传感器测定甲胎蛋白

Study on the determination of Edifenphos in grain by HPLC-MS/MS

通过原位合成法以金纳米种子作为母核诱导纳米金在其表面原位生长生成爆米花型金纳米粒子,标记甲胎蛋白抗体(anti-AFP)和辣根过氧化物酶(HRP)结合形成免疫复合物(antiAFP@popcorn-shaped GNP@HRP)作为检测抗体。氧化石墨烯-亚甲基蓝-金纳米粒子(GOM B-AuNPs)纳米复合材料滴涂到玻碳电极(GCE)表面,形成纳米复合物用于捕获甲胎蛋白抗体构建了免疫传感平台(anti-AFP/GO-MB-AuNPs/GCE)。实验采用夹心型免疫分析模式,功能化爆米花型金纳米材料与石墨烯纳米复合物呈现出良好的性能,建立了一种可行的电流型免疫分析法用于灵敏分析血清样品中甲胎蛋白AFP。在优化的实验条件下,免疫传感器DPV电流响应与AFP浓度的对数值呈正比,线性范围为0.001~20 ng/mL,检测限为0.31 pg/mL(S/N=3)。制备的免疫传感器有良好的精密度,选择性和稳定性,可初步应用于临床检验中AFP测定。

A class of popcorn shape of gold nanoparticle as molecular tags was developed to immobilize anti-AFP and HRP for sensitive electrochemical immunoassay on the prepared graphene oxide- methylene blue-gold nanoparticles （GO-MB-AuNPs） nanocomposites sensing platform for determination of alpha-fetoprotein （AFP）. To construct such a molecular tag, gold nanoparticle seeds as nuclear parent induced nanogolds in situ growth on the surface to form popcorn shape of gold nanoparticles, and then, horseradish peroxidase and anti-AFP were conjugated onto popcorn shape of gold nanoparticles. A sandwich-type immunoassay format was employed for determination of AFP. Under optimal conditions, the response DPV signal and the logarithm of AFP concentration, from 0. 001 ng/mL to 20 ng/mL, had a linear relationship, and the detection limit was 0. 32 pg/mL （SIN = 3）. The immunesensor had good precision, selectivity and stability, and was applied to the serum sample analysis, which indicated the as-prepared immunosensor could be preliminary applied to clinical examination.