西南区应用的7个玉米骨干系与美国自交系的SSR标记分析

Analysis of SSR markers in seven southwest corn backbone inbred lines and 49 United States corn inbred lines

利用41对玉米SSR引物对49份美国玉米自交系和7份西南区骨干自交系进行遗传多样性分析,共检测出169个等位基因有变异位点,每对引物检测到等位基因2~10个,平均4.12个;每对SSR引物的多态信息量(PIC值)变化范围为0.299~0.885,平均值为0.652。按UPGMA方法对供试自交系进行聚类,以遗传距离0.572为基准,可将供试材料划分为七类。56份自交系的SSR标记聚类分析表明,78%的美国自交系与西南区常用自交系之间也存在着较大的遗传差异,被单独聚为四类,而22%美国玉米自交系与西南区玉米自交系具有一定的同源性,被聚在第Ⅰ、Ⅳ和Ⅵ类。通过美国玉米种质和西南区应用种质的SSR标记分析,为加强西南区玉米育种材料的改良、创新和利用,以及品种选育提供参考。

41 SSR primers were applied to analyze genetic diversity in 49 U. S. corn inbred lines and 7 south-west backbone inbred lines. A total of 169 alleles were detected with an average of 4. 12 per SSR primer pair, ranging from 2 - 10. The polymorphism information content （ PIC ） of SSR primers ranged from 0. 299 to 0. 885, with a mean of 0. 652. According to the UPGMA method, the test inbred lines could be divided into seven groups by clustering with the genetic distance of 0. 572 as the benchmark. Clustering analysis of SSR markers for 56 inbred lines showed that 78% of U. S. corn inbred lines and southwest backbone inbred lines had significant genetic differences, which were clustered into four groups. While 22% of U. S. corn inbred lines and southwest backbone inbred lines had certain homologous origin, which were clustered into three （ I , IV and VI ） groups. Through the analysis of SSR marker of U. S. corn germplasm and southwest maize germplasm, the study can provide the reference for the improvement, innovation and utilization of maize breeding material in southwest China.