70周龄长顺绿壳蛋鸡HO-1基因在不同组织中表达差异研究

Study on Different Expression of HO-1 Gene of 70-weeks-old Changshun Blue-shelled Chicken in Different Tissues

为研究HO-1基因在70周龄长顺绿壳蛋鸡不同组织中的表达差异,选取70周龄长顺绿壳蛋鸡9只(每种基因型3只),采集每只鸡的大脑、肝脏、肾脏、卵巢、输卵管和子宫组织,提取其总RNA进行反转录,利用实时荧光定量PCR方法检测样品中HO-1基因的表达水平,比较同一组织不同基因型及同一基因型不同组织间HO1基因mRNA的表达差异。结果表明,在同一组织不同基因型及同一基因型不同组织间HO-1基因的表达存在差异;其中HO-1基因在肝脏中表达量最高,且3种基因型间肝脏表达量存在极显著差异(P<0.01);白壳蛋鸡子宫中HO-1基因表达量极显著高于其他两种(P<0.01),纯合绿壳蛋鸡与杂合绿壳蛋鸡无显著差异(P>0.05)。

In order to study the different expression of HO-1 gene in different tissues of 70-weeks-old Changshun Blue-shelled chickens. We collected nine （3 of each genotype） Changshun Blue-shelled chickens of 70-weeks-old as samples. The brain, liver, kidney, ovary, fallopian tube and uterus were collected, and total RNA of every tissue was extracted and reversed transcription to cDNA.The expression of HO-1 mRNA in different samples were detected by quantitative Real-time PCR.We compared the expression in the same tissues of different genotypes and in the different tissues of the same genotype. The results suggested that there were differences among different tissues of the same genotypes and different genotypes of the same tissues. The expression of HO-1 gene was highest in the liver, and there were significant difference in different genotype （P〈 0.01）.The expression level in the uterus of White-shelled chicken was extremely significantly higher than other two genotypes （P〈 0.01）,there was no significant difference between homozygous and heterozygous Blue-shelled chickens （P〉 0.05）.