麦粒灸对Lewis肺癌小鼠炎性微环境中白介素-6及信号转导蛋白和转录激活因子3的影响

Effect of Grain-moxibustion on IL-6 and STAT 3 in Inflammatory Microenvironment of Lewis Lung Cancer Mice

目的:观察麦粒灸对Lewis肺癌小鼠炎性微环境中血清白介素-6(IL-6)及瘤体信号转导蛋白和转录激活因子3(STAT 3)的影响,探讨麦粒灸对Lewis肺癌的作用机制。方法:将C 57BL/6小鼠随机分为空白组、模型组和治疗组,每组10只。皮下注射Lewis肺癌细胞悬液建立Lewis肺癌小鼠模型。成瘤后治疗组予以麦粒灸治疗,取双侧"后三里"穴,每穴5壮,1次/d,共10d。观察各组小鼠的体质量、生存状态积分、瘤体体积和生存率,采用ELISA法检测血清IL-6的表达水平,采用Western blot法检测瘤体中STAT 3的表达水平。结果:与空白组比较,模型组小鼠体质量明显升高(P<0.05)。模型组和治疗组小鼠瘤体体积逐渐增大,治疗8、10d时治疗组小鼠瘤体体积明显小于模型组(P<0.05)。模型组小鼠生存状态恶化、生存率降低,治疗6、8、10d时治疗组小鼠生存状态积分明显高于模型组(P<0.05)。与空白组比较,模型组小鼠血清IL-6和瘤体中STAT 3表达水平均明显升高(P<0.001);与模型组比较,治疗组小鼠血清IL-6和瘤体中STAT 3表达水平均明显下降(P<0.001)。结论:麦粒灸可提高Lewis肺癌小鼠生存状态,降低死亡率,控制瘤体生长速度,其机制可能与调节肿瘤炎性微环境、抑制信号因子IL-6及STAT 3持续活化有关。

Objective To observe the effect of grain-moxibustion on serum interleukin-6（IL-6） and signal transducers and activators of transcription 3 （STAT 3） in inflammatory microenvironment of Lewis lung cancer mice, so as to explore its possible mechanism underlying relieving Lewis lung cancer. Methods C 57 BL/6 mice were randomly divided into blank control group, model group, and moxibustion group （n = 10 in each group）. Lewis lung cancer model was established by hypodermic injection of Lewis tumor cell suspension. Mice in the moxibustion group were treated by grain-moxibustion at bilateral ＂Housanli＇（ST 36）, 5 zhuang each acupoint, once a day for 10 days. The body weight, survival state score, volume of tumor, and survival rate were observed. Serum IL-6 level was detected by ELISA, and the expression of STAT 3 in tumor tissue was determined by Western blot. Results Compared with the blank control group, the body weight was significantly increased in the model group （ P〈0.05）. The volume of tumor increased significantly in the model group, while it markedly decreased in the moxibustion group after 8 and 10 days treatment compared with the model group （ P〈0.05）. The survival state of mice deteriorated companied with decreased survival rate in the model group, however, after 6, 8 or 10 days treatment, the survival state scores were increased in the moxibustion group than in the model group （P〈0.05）. Compared with the blank control group, both serum IL-6 level and STAT 3 expression in tumor tissue were increased significantly in the model group （ P〈0. 001 ）. Compared with the model group, both serum IL-6 level and STAT 3 expression in tumor tissue were decreased significantly in the moxibustion group （P〈0. 001 ）. Conclusion Grain-moxibustion has remarkable effect on Lewis lung cancer, by improving survival state, reducing death rate, and controlling growth rate of tumor, which might be associated with its function of inhibiting the activation of IL-6 and STAT 3 in inflammatory microenvironment.