点灸、电针对糖尿病胃轻瘫大鼠胃窦内皮细胞型一氧化氮合酶及血管紧张素ⅡmRNA表达的影响

Effect of Point-moxibustion and Electroacupuncture on the Expression of Endothelial Nitric Oxide Synthase mRNA and Angiotensin 2 mRNA in Gastric Antrum in Diabetic Gastroparesis Rats

目的:观察点灸、电针对糖尿病胃轻瘫(DGP)大鼠胃肠运动和内皮细胞型一氧化氮合酶(eNOS)、血管紧张素Ⅱ(ATⅡ)mRNA表达的影响,探讨点灸、电针治疗DGP的效应差异及作用机制。方法:SD大鼠随机分为空白组、模型组、点灸组、电针组,每组10只。采用腹腔注射2%链脲佐菌素配合高脂高糖饮食建立DGP大鼠模型。点灸组和电针组选取"足三里""三阴交""梁门"穴,点灸组于0、10、20min对穴位进行点灸,电针组电针20min,每日治疗1次,治疗15d。检测各组大鼠血糖、胃排空率和小肠推进率,ELISA法检测血浆内皮素1(ET-1)含量,Real-time PCR法检测胃窦部组织eNOS mRNA、ATⅡmRNA的表达。结果:与空白组比较,模型组血糖明显升高(P<0.01);与模型组比较,点灸组和电针组血糖明显降低(P<0.05)。与空白组比较,模型组胃排空率及小肠推进率显著降低(P<0.01),血浆ET-1含量显著升高(P<0.01),胃窦部eNOS mRNA表达显著降低、ATⅡmRNA表达显著升高(P<0.01)。与模型组比较,点灸组与电针组胃排空率及小肠推进率显著升高,血浆ET-1含量显著降低,胃窦部eNOS mRNA表达显著升高、ATⅡmRNA表达显著降低(P<0.05)。点灸组与电针组之间比较差异无统计学意义(P>0.05)。结论:点灸与电针均可有效促进DGP大鼠胃肠运动,改善胃排空迟缓症状,两种疗法疗效相当,其作用机制可能与升高胃窦部eNOS mRNA表达、降低ATⅡmRNA表达有关。

Objective To observe the effect of point-moxibustion and electroacupuncture（EA） on gastrointestinal motility, and expression of endothelial nitric oxide synthase（eNOS） mRNA and angiotensin 2（ATⅡ ） mRNA in gastric antrum in diabetic gastroparesis（DGP） rats, so as to reveal the different effect between point-moxibustion and EA, and explore the mechanism underlying improvement of DGP. Methods Forty SD rats were randomly divided into normal, model, point-moxibustion and EA groups, 10 rats in each group. The DGP model was established by intraperitoneal injection of streptozotocin （STZ, 2%, 55 mmol/kg） and fed with high-sugar and high-fat fodder. ＂Zusanli＂ （ST 36）, ＂Sanyinjiao＂ （SP 6） and ＂Liangmen＂ （ST 21 ） were chosen to be stimulated with moxibustion at 0, 10 and 20 min in the point-moxibustion group or with EA for 20 min in the EA group. The treatment was conducted once daily for 15 days. After treatment, blood glucose, gastric emptying rate and small intestinal propulsive rate were measured. The plasma endothelin 1 （ET-1） content was detected by ELISA. The mRNA expression of eNOS and AT Ⅱ in gastric antrum were detected by Real-time PCR. Results Compared with the normal group, the blood glucose in the model group was significantly higher （P〈0.01）. Compared with the model group, the blood glucose decreased significantly in the point-moxibustion group and EA group （P〈0.05）. In comparison with the normal group, gastric emptying rate and small intestinal propulsive rate were decreased, the content of plasma ET-1 was increased, the expression of eNOS mRNA in gastric an- trum was significantly reduced and the expression of AT IT mRNA was significantly increased in the model group（ P〈0. 01 ）. In comparison with the model group, gastric emptying rate and small intestinal propulsive rate were increased, the content of plasma ET-1 was decreased, the expression of eNOS mRNA in gastric antrum was higher and the expression of AT Ⅱ mRNA was significantly lower in both point-moxibustion group and EA group （P〈0.05）, and there were no significant differences between the two treatment groups in above mentioned indexes （P〉0.05）. Conclusion Both point-moxibustion and EA can effectively promote gastrointestinal motility in DGP rats and improve the symptoms of delayed gastric emptying. The two therapies are effective, and their mechanism may be related to the increase of eNOS mRNA expression and the decrease of AT Ⅱ mRNA expression in gastric antrum.