抗-M抗体引起的血型鉴定困难分析及配血影响

The analysis of blood typing difficulties caused by the anti-M

目的 探讨抗-M抗体干扰血型鉴定及其对配血的影响.方法 血型及抗体鉴定按照血型血清学试验操作标准操作.37 ℃氯化钠注射液洗涤4次后用试管法做正定型,洗涤后的红细胞加等量自身血清在4℃吸收≥4 h,离心后做反定型.确定抗体性质用2-Me破坏IgM型 抗体前后的血清标本进行抗体鉴定红细胞检测,并进一步用自制NN型反定型红细胞检测患者血清.配血选择与受血者ABO同型的MM型和NN型红细胞,依次采用盐水法,凝聚胺法,抗人球蛋白介质法进行配血试验.结果 NN型的反定型细胞正反定型一致,患者标本与抗-M不凝集,与抗-N则均凝集.患者与红细胞上存在M抗原的供血者进行常规交叉配血时,卡式法均有凝集,而当选择不含有M抗原的红细胞,配血均相合.结论 患者血清中存在的影响血型鉴定的抗-M抗体.

Objective To investigate the anti-M interference the blood group identification and its effect on blood matching test.Methods Standard serological test to test the Blood group and the antibody.First,The red cells,washed with 37 ℃ of physiological saline four times,test the positive typing,then add equal volume of auto-serum to the rest red cells,4 ℃ absorption ≥4 hours,after centrifugation for the reverse typing;The specimens with the irregular antibody,antibody identification RBC to test the specific antibody,then 2-Me to damage the IgM to detect the IgG.self-made NN type negative stereotypes cell to test the Serum samples.Select the RBC,MM or NN type,which have the same ABO type with the recipients to do the cross-match test.Results NN type of negative typing cell detection consistent the positive and negative blood group.All the specimens reacted with anti-N and form agglutination（intensity ＋＋）,and the anti-M not.The RBC,with M antigen,agglutinated with the specimens,while without M antigen not.Conclusions The antibody in serum of patients,which effect the blood typing,was anti-M.