积雪草苷抗大鼠免疫性肝纤维化作用研究

Study on Effects of Asiaticoside Against Hepatic Fibrosis in Rats

目的研究积雪草有效成分积雪草苷的抗纤维化作用。方法建立猪血清致大鼠免疫性肝纤维化模型,设正常组、模型组、γ干扰素组、积雪草苷高、中、低剂量组(0.9,0.3,0.1 g·kg^(-1)),检测大鼠血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)以及肝组织中羟脯氨酸(Hyp)的含量,HE及Masson染色观察肝组织病理学变化,并且对纤维化程度进行半定量评分。结果积雪草苷能显著降低大鼠血清中的ALT、AST水平以及肝组织中的Hyp水平;积雪草苷各剂量组均能减轻大鼠肝组织的纤维化程度,其中高剂量组尤为显著(P<0.01)。结论积雪草苷具有抗免疫性大鼠的肝纤维化作用,且呈现出一定的量效关系。

OBJECTIVE To research the effect and mechanism of asiaticoside from Centella asiatica （L.） Urban on rats with hepatic fibrosis. METHODS Hepatic fibrosis model was induced by intracutaneous injection of swine serum. The rats were divided into normal group, model group, γ-interferon group, and asiaticoside group with the dose of 0.9, 0.3, 0.1 g·kg-1. The content of alanine aminotransferase（ALT） and aspartate aminotransferase（AST） in serum and liver hydroxyproline（Hyp） from hepatic tissue were detected. The pathological change of hepatic tissue were observed by HE and Masson staining, and the extent of fibrosis by semi quantitative score were evaluated. RESULTS The levels of ALT, AST in serum and Hyp in liver tissue were decreased obviously by asiaticoside. All asiaticoside groups could reduce the extent of fibrosis, significantly in the high dose group（P〈0.01）. CONCLUSION Asiaticoside has the raputic effect on immune hepatic fibrosis which shows a dose-dependent manner.