梭梭人工接种肉苁蓉与野生肉苁蓉活性成分及金属元素的含量测定

Comparative Study on the Effective Components and Contents of Metallic Elements of Manual Cultivation Cistanch Deserticola Parasitized by Haloxylon Ammodendron and Wild Cistanch Deserticola

目的研究梭梭人工接种肉苁蓉与野生肉苁蓉药典指标成分及金属元素的含量差异。方法分别应用HPLC和空气-乙炔火焰原子吸收光谱法进行活性成分及金属元素测试,并利用SPSS统计分析软件进行数据处理。结果野生样品中毛蕊花糖苷的平均含量(2.58±0.45)%高于梭梭人工接种品(1.13±0.37)%,差异有统计学意义(P<0.05),两者松果菊苷含量无显著差异。此外野生样品中金属元素Na、K、Ca含量高于梭梭人工接种品,且差异有统计学意义(P<0.05),其余元素差异不显著。结论梭梭人工接种肉苁蓉与野生肉苁蓉的成分含量具有一定的差异性。

OBJECTIVE To investigate the differences of the effective components contents and metallic elements between manual cultivation Cistanch deserticola parasitized by Haloxylon ammodendron and wild Cistanch deserticola. METHODS HPLC and atomic absorption spectrometry with air-acetylene flame methods were used to determine the content of effective components and metallic elements, and these results were analyzed by SPSS statistical analysis software. RESULTS The content of acteoside （2.58±0.45）% in wild samples was significantly higher than that in manual cultivated samples （1.13±0.37）% （P〈0.05）, the content of echinacoside had no significant difference between manual cultivation and wild samples. Moreover, the content of metallic elements Na, K, Ca in the wild samples were significantly higher than those in the cultivated samples （P〈0.05）. CONCLUSION Wild and manual cultivated samples have differences in the contents of components.