刺五加苷B对仔猪小肠上皮细胞紧密连接蛋白及细胞因子mRNA表达量的影响

Effects of Eleutheroside B on Expression of Tight Junction Protein and Inflammatory Cytokine mRNA in Small Intestinal Epithelial Cells of Piglets

以仔猪小肠上皮细胞为模型,应用实时荧光定量PCR方法测定相关基因指标,研究刺五加苷B(EB)对仔猪小肠上皮(IPEC-J2)细胞紧密连接蛋白及炎性细胞因子mRNA表达量的影响。结果表明:0.1 mg/m L刺五加苷B显著增强肠道细胞紧密连接蛋白Occludin、Claudin-3、ZO-1和抗炎性细胞因子IL-10和TGF-β的mRNA表达量,且减弱促炎性细胞因子IL-6、TNF-α、INF-γ的mRNA表达量。说明刺五加苷B可通过改变细胞间紧密连接蛋白和细胞因子的表达从而对维持肠道屏障功能的完整性起到促进作用,有益于仔猪肠道的健康发育。

In this experiment,small intestinal epithelial cells of piglets were used as the model to study the effects of eleutheroside B （EB） on mRNA expression of cell tight junction proteins and inflammatory cytokines in small intestinal epithelial cells of piglets （IPEC-J2） cells by qRT-PCR method.The results showed that eleutheroside B significantly enhanced the expression of mRNA in intestinal tight junction proteins including Claudin-3,Occludin and ZO-1,and anti-inflammatory cytokines including IL-10 and TGF-β.At the same time,eleutheroside B decreased the expression of mRNA in pro-inflammatory cytokines including IL-6,TNF-α and INF-γ.The results showed that eleutheroside B could improve intestinal barrier function of piglets by changing the expression of tight junction proteins and cytokines.