日本结缕草感染立枯丝核菌初期NCED基因的表达及响应分析

The analysis of NCEDgene responing to Rhizoctonia solani early invasion in Japan zoysia

以转录组数据为基础,筛选并克隆出"Zenith"品种日本结缕草NCED基因片段。通过荧光定量PCR手段获得NCED基因在立枯丝核菌根部侵染初期(0~48h)表达变化模式。结果表明,胁迫处理后NCED基因表达量比未感染时均升高,且随时间变化呈现先上升后下降的趋势,在36h大量表达达到最高。进一步检测植物根部内源ABA水平发现,0~48hABA水平变化趋势与NCED基因表达模式趋势一致,并在48h之后趋于平衡。结果表明,该基因调控植物内源ABA的合成并进一步参与植物抗病机制。本研究为探究结缕草的抗病分子响应机制及育种方面的研究提供理论依据。

Part of conservative sequence of Japan zoysia＇Zenith＇ NCED gene was screened and cloned on the basis of the transcriptome. Time-space expression pattern of NCED gene was studied under Rhizoctonia solani early invasion（0-48 h）in ＇Zenith＇ roots through real-time quantitative PCR. Results indicated that the NCED gene expression after the stress treatment was higher than that without infection, and showed an increasing tendency before the decrease,the gene expression achieving the highest at 36 h. Further test of ABA level in＇ Zenith＇ roots showed the same trend with NCED gene expression from 0-48 h and flattened ou,t after 48 h. It suggested that NCED gene regulated the synthesis of phytohormone ABA and was probably further involved in plant disease resistance. This paper provides a theoretical basis for Japan zoysia resistance mechanism and breeding research.