脂肪移植治疗DNCB诱导ACD模型小鼠的实验研究

Research on fat transplantation treatment for DNCB-sensitized allergic contact dermatitis of mouse model

目的探究脂肪移植治疗2,4-二硝基氯苯(DNCB)诱导变应性接触性皮炎(allergic contact dermatitis,ACD)模型小鼠的效果及其可能机制。方法采用DNCB复制ACD小鼠模型,随机分成A(脂肪移植治疗组)、B(假手术治疗组)、C(药物治疗组)、D组(自然修复组),观察脂肪移植对ACD小鼠的治疗效果。2周后处死小鼠,取背部皮损处全层皮肤组织,苏木精-伊红染色法染色观察各组小鼠皮肤组织病理改变;实时荧光定量聚合酶链反应(q RT-PCR)检测皮损中白介素10(IL-10)、白介素17(IL-17)、干扰素-γ(IFN-γ)的表达。结果 A、C组可以观察到表皮厚度恢复正常,角质层变薄;B、D组无明显改变。RT-PCR检测发现A、C组皮肤组织中IL-10和IL-17 m RNA水平低于D组,IFN-γm RNA较D组升高(P<0.05),而B与D组比较差异无统计学意义(P>0.05)。结论脂肪移植可降低皮损中IL-10和IL-17的表达,促进IFN-γ产生,减轻皮损炎症。

Objective To explore whether fat transplantation can improve the symptoms of the mouse model of allergic contact dermatitis （ACD） induced by dinitroehlorobenzene （DNCB） as well as its possible mecha- nism. Methods ACD mouse models were randomly divided into group A （fat transplantation group）, group B （pseudo surgery group）, group C （medication group） and group D （spontaneously-recovered model group）. The HE-stained skin tissues of each group were observed under light microscope. The expression levels of IL-10, IL-17 and IFN-Y mRNAs in the skin lesions were detected by qRT-PCR. Results In the groups A and C, the horny layers became thinner, and the thickness of epidermis recovered to normal while there were no overt changes in the groups B and D. The expression levels of IL-10 and IL-17 mRNAs in the skin tissues of the groups A and C were clearly lower than those of the group D, the level of IFN-＇y mRNA was higher than that of the group D （P〈 0.05）; however, there was no significant changes in the IL-10, IL-17 or IFN-Y mRNA level between the groups B and D. Conclusions Fat transplantation can decrease the expression levels of IL-10 and IL-17 in skin lesions, promote the production of IFN-Y, reduce the symptoms of skin lesions and promote the recovery of skin lesions in the mouse model.