诱导子对蜀葵悬浮细胞、不定根培养的影响

Effects of Elicitors on Uronic Acid and the Total Flavonoid Content in Alcea rosea Suspension Cells and Adventitious Root

为探究诱导子对蜀葵(Alcea rosea)细胞次生代谢产物的影响,分别使用茉莉酸甲酯、水杨酸、酵母提取物3种诱导子处理快速生长期后期的蜀葵悬浮细胞.结果表明:茉莉酸甲酯和水杨酸均会抑制悬浮细胞和不定根的生长,而低浓度的酵母提取物则会促进其生长;茉莉酸甲酯对于悬浮细胞和不定根中的糖醛酸含量没有影响,而水杨酸和酵母提取物均对其糖醛酸含量有促进作用,且酵母提取物效果更好;3种诱导子均能有效提高悬浮细胞和不定根中的总黄酮含量,其中以茉莉酸甲酯效果最好,水杨酸次之.这些结果表明,3种诱导子对蜀葵悬浮细胞和不定根培养物都能产生影响,但综合考虑酵母提取物的效果最为明显,水杨酸次之.

In order to explore the influence of elicitors on secondary metabolites of Alcea rosea cells, methyl jasmonate, salicylic acid and yeast extracts were used to treat Alcea rosea suspension cells and adventitious roots. The results showed that methyl jasmonate and salicylic acid inhibited the growth of suspension cells and adventitious roots, but low concentra- tion of yeast extracts were beneficial to their growth. While methyl jasmonate had no effect on the uronic acid content in both cultures, salicylic acid and yeast extracts improved their uronic acid contents, and yeast extracts functioned better. In addi- tion, the total flavonoid contents were effectively improved by these elicitors, and methyl jasmonate was the best, followed by salicylic acid. These results concluded that during Alcea rosea suspension cells and adventitious roots culturing in vitro, all the three elicitors have effect on cultures and the secondary metabolites in some degree, but the best was yeast extracts, followed by salicylic acid.